Mouse Anti-Mfap4 Recombinant Antibody (clone HG-HYB 7-1) (CAT#: HPAB-0488-FY)

This product is a recombinant antibody that recognizes Human Mfap4. The monoclonal antibody HG-HYB 7-1 reacts with the epitope of Mfap4.

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Inhib

Figure 1 shows MFAP4 interaction with smooth muscle cells is inhibited by MFAP4 blocking antibodies and induced VSMC migration is inhibited by MFAP4 blocking anti-bodies in vitro.

fHAoSMCs were seeded in poly-D-lysine (PDL, negative control), or rMFAP4 coated micro-well plates with a central rounded area blocked by a cell stopper. The number of flourescently labelled cells was counted within this central area of the well (depicted) twenty hrs after removal of the cell stoppers and ± the anti-MFAP4 antibodies HG-HYB 7-5 (7-5), HG-HYB 7-14 (7-14), HG-HYB 7- 18 (7-18), or isotype control antibodies (iso) in order to quantitate cellular migration

FuncS

Figure 2 Asthmatic BSMC proliferation.

Asthmatic primary bronchial smooth muscle cell (BSMC) proliferation as measured by absorption (OD590) were measured against treatment with varying HG-HYB 7-1 concentrations.

FuncS

Figure 3 CD45 staining of macrophages shows a decrease in macrophage infiltration with aMFAP4 or aVEGF-A treatment.

FuncS

Figure 4 Fundus fluorescein angiography at day 14 shows a decrease in lesion size and lesion density in eyes treated with aMFAP4 or aVEGF-A compared to IgG treated eyes.

Representative images of lesions from eyes treated with IgG, aVEGF-A and aMFAP4 at 1 μg and 5μg (A). aMFAP4 at 1 μg and 5μg is able to reduce both average lesion size (B) and average integrated density of lesion (C) compared to IgG controls.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human Mfap4
  • Species Reactivity
  • Human
  • Clone
  • HG-HYB 7-1
  • Applications
  • ELISA, WB, FuncS, IF, Inhib
  • Related Disease
  • Cancer

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Functional Assay, IF, Inhibition.

Target

  • Alternative Names
  • Mfap4; Microfibrillar-associated protein 4

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "Mfap4"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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