Mouse Anti-MFGE8 Recombinant Antibody (clone 2B2D10)
CAT#: ZG-0144U
Mouse anti-MFGE8 antibody (clone 2B2D10) displayed high specificity against the MFGE8 protein of Human.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-MFGE8 Antibody (ZG-0144U) in IHC
Immunohistochemical of paraffin-embedded human Colon cancer tissues using ZG-0144U at dilution of 1:200.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2807/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2807/8016_A_1_7_selected.jpg
Normal Tissue
Figure 3 IHC staining of human endometrium
High expression. RNA expression: 444.7 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_B_5_3_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Low expression. RNA expression: 21.2 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_9_2_val_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2807/8042_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Enterocytes - Microvilli
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (microvilli)
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2807/8016_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_7_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140545-MFGE8
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Specifications
- Immunogen
- Recombinant Human MFGE8 protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human MFGE8
- Species Reactivity
- Human
- Clone
- 2B2D10
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
- Related Disease
- Anal Paget's Disease and Topographical Agnosia
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user.
IHC:1:50-1:500
Target
- Alternative Names
- BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888; lactadherin; O-acetyl disialoganglioside synthase; breast epithelial antigen BA46; medin; milk fat globule-EGF factor 8 protein; sperm associated antigen 10; sperm surface protein hP47
- Gene ID
- 4240
- UniProt ID
- Q08431
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-657 | Recombinant Human Anti-human MFGE8 Antibody | IF, ELISA, FuncS | IgG |
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| FAMAB-0225CQ | Mouse Anti-MFGE8 Recombinant Antibody (clone Mc3) | ELISA, IF, Block, FuncS | Mouse IgG1, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-657-F(E) | Recombinant Human Anti-human MFGE8 Antibody Fab Fragment | WB, ELISA, IHC, FuncS | Fab |
| FAMAB-0225CQ-F(E) | Mouse Anti-MFGE8 Recombinant Antibody (clone Mc3); Fab Fragment | ELISA, IF, FuncS | Mouse Fab |
| FAMAB-0365JF-F(E) | Mouse Anti-MFGE8 Recombinant Antibody (clone 3D10); Fab Fragment | IB, WB, ELISA, FuncS | Mouse Fab |
| HPAB-2103-FY-F(E) | Human Anti-MFGE8 Recombinant Antibody (clone HuHMFG1); Fab Fragment | IA | Humanized Fab |
| HPAB-2104-FY-F(E) | Mouse Anti-MFGE8 Recombinant Antibody (clone HMFG1); Fab Fragment | IA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-657-S(P) | Recombinant Human Anti-human MFGE8 Antibody scFv Fragment | WB, IP, FuncS | scFv |
| FAMAB-0225CQ-S(P) | Mouse Anti-MFGE8 Recombinant Antibody (clone Mc3); scFv Fragment | ELISA, IF, FuncS | Mouse scFv |
| FAMAB-0365JF-S(P) | Mouse Anti-MFGE8 Recombinant Antibody (clone 3D10); scFv Fragment | IB, WB, ELISA, FuncS | Mouse scFv |
| HPAB-2103-FY-S(P) | Human Anti-MFGE8 Recombinant Antibody (clone HuHMFG1); scFv Fragment | IA | Humanized scFv |
| HPAB-2104-FY-S(P) | Mouse Anti-MFGE8 Recombinant Antibody (clone HMFG1); scFv Fragment | IA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0763CL-S(P) | Mouse Anti-MFGE8 Recombinant Antibody; scFv Fragment (TAB-0763CL-S(P)) | ELISA | Mouse scFv |
| TAB-0764CL-S(P) | Mouse Anti-MFGE8 Recombinant Antibody; scFv Fragment (TAB-0764CL-S(P)) | ELISA | Mouse scFv |
| TAB-0765CL-S(P) | Mouse Anti-MFGE8 Recombinant Antibody; scFv Fragment (TAB-0765CL-S(P)) | ELISA | Mouse scFv |
| TAB-0766CL-S(P) | Mouse Anti-MFGE8 Recombinant Antibody; scFv Fragment (TAB-0766CL-S(P)) | ELISA | Mouse scFv |
| TAB-414MZ-F(E) | Anti-Human MFGE8 Recombinant Antibody Fab Fragment (CTMO1) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0768CL-S(P) | Human Anti-MFGE8 Recombinant Antibody; scFv Fragment (TAB-0768CL-S(P)) | ELISA | Humanized scFv |
| TAB-0769CL-S(P) | Human Anti-MFGE8 Recombinant Antibody; scFv Fragment (TAB-0769CL-S(P)) | ELISA | Humanized scFv |
| TAB-0770CL-S(P) | Human Anti-MFGE8 Recombinant Antibody; scFv Fragment (TAB-0770CL-S(P)) | ELISA | Humanized scFv |
| TAB-0771CL-S(P) | Human Anti-MFGE8 Recombinant Antibody; scFv Fragment (TAB-0771CL-S(P)) | ELISA | Humanized scFv |
| TAB-415MZ-F(E) | Anti-Human MFGE8 Recombinant Antibody Fab Fragment (Hu:CTMO1) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC048 | PE-A*02:01/Human BA46 (GLQHWVPEL) MHC Tetramer | FCM | |
| MHC-LC049 | APC-A*02:01/Human BA46 (GLQHWVPEL) MHC Tetramer | FCM | |
| MHC-LC050 | BV421-A*02:01/Human BA46 (GLQHWVPEL) MHC Tetramer | FCM | |
| MHC-YF323 | A*02:01/Human BA46 (NLFETPVEA) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0365JF | Mouse Anti-MFGE8 Recombinant Antibody (clone 3D10) | WB, ELISA, IHC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4370 | Anti-MFGE8 Immunohistochemistry Kit | IHC |
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