Mouse Anti-MFGE8 Recombinant Antibody (clone 2B2D10)

Name: Mouse Anti-MFGE8 Recombinant Antibody (clone 2B2D10)
SKU: ZG-0144U
Rating: 5 (1 reviews)

CAT#: ZG-0144U

Mouse anti-MFGE8 antibody (clone 2B2D10) displayed high specificity against the MFGE8 protein of Human.

Tested Data Gene Expression

IHC

Figure 1 Mouse Anti-MFGE8 Antibody (ZG-0144U) in IHC

Immunohistochemical of paraffin-embedded human Colon cancer tissues using ZG-0144U at dilution of 1:200.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2807/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human placenta

Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2807/8016_A_1_7_selected.jpg

Normal Tissue Figure 3 IHC staining of human endometrium

High expression. RNA expression: 444.7 nTPM

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_B_5_3_val_selected.jpg

Normal Tissue Figure 4 IHC staining of human duodenum

Low expression. RNA expression: 21.2 nTPM

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_9_2_val_selected.jpg

Normal Tissue Figure 5 Cerebral cortex

Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2807/8042_B_7_5.jpg

Normal Tissue Figure 6 Colon

Enterocytes - Microvilli
Staining: High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_7_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_8_4.jpg

Normal Tissue Figure 8 Kidney

Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (microvilli)
Staining: High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_7_5.jpg

Normal Tissue Figure 9 Testis

Cells in seminiferous ducts
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2807/8016_A_4_6.jpg

Normal Tissue Figure 10 Lymph node

Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2753/7591_A_7_8.jpg

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166, HSkMC)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140545-MFGE8

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Specifications

Immunogen
Recombinant Human MFGE8 protein

Host Species
Mouse

Type
Mouse IgG2b

Specificity
Human MFGE8

Species Reactivity
Human

Clone
2B2D10

Applications
ELISA, IHC

Conjugate
Unconjugated

Related Disease
Anal Paget's Disease and Topographical Agnosia

Product Property

Purification
Protein G purified

Purity
>95% as determined by SDS-PAGE

Concentration
See the COA

Buffer
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.

Preservative
0.03% Proclin 300

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user.
IHC:1:50-1:500

Target

Alternative Names
BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888; lactadherin; O-acetyl disialoganglioside synthase; breast epithelial antigen BA46; medin; milk fat globule-EGF factor 8 protein; sperm associated antigen 10; sperm surface protein hP47

Gene ID
4240

UniProt ID
Q08431

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0144U.pdf

MSDS

MSDS - ZG-0144U.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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