Mouse Anti-mGluR Recombinant Antibody (clone mGluR7) (CAT#: HPAB-0335LY)

Figure 1 GLUT1, VGLUT2, VGAT, mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 antibodies recognized bands of 55, 60, 57, 140, 132, 100,and 190, 97, 100, and 132 kDa in the order in crude membrane fractions of rat cerebral cortex.

Bragina, L., Bonifacino, T., Bassi, S., Milanese, M., Bonanno, G., & Conti, F. (2015). Differential expression of metabotropic glutamate and GABA receptors at neocortical glutamatergic and GABAergic axon terminals. Frontiers in cellular neuroscience, 9, 345.

Figure 2 Expression of metabotropic glutamate and GABA receptors in cerebral cortex.

The figure shows representative images from the VGLUT1 series(first row), the VGLUT2 series (second row), and the VGAT series (third row). Puncta were considered double-labeled (arrows) when overlap was virtually complete orwhen a given punctum was entirely included in the other puncta.

Bragina, L., Bonifacino, T., Bassi, S., Milanese, M., Bonanno, G., & Conti, F. (2015). Differential expression of metabotropic glutamate and GABA receptors at neocortical glutamatergic and GABAergic axon terminals. Frontiers in cellular neuroscience, 9, 345.

Figure 3 An mGluR7 agonist increases spinal nocifensive reflexes of normal animals whereas anmGluR8 agonist inhibits nocifensive reflexes of arthritic animals.

Hindlimb withdrawal thresholds in response to compression of the knee joint were measuredin normal animals and in animals with a knee joint arthritis (5–6 h postinduction. Arthritic animals had lower withdrawal thresholds than normal animals, reflectingmechanical hypersensitivity.

Palazzo, E., Fu, Y., Ji, G., Maione, S., & Neugebauer, V. (2008). Group III mGluR7 and mGluR8 in the amygdala differentially modulate nocifensive and affective pain behaviors. Neuropharmacology, 55(4), 537-545.

Figure 4 An mGluR7 agonist increases audible vocalizations of normal animals whereas anmGluR8 agonist inhibits audible vocalizations of arthritic animals.

Audible vocalizations evoked by innocuous (500 g/30 mm2) and noxious (2000 g/30 mm2)compression of the knee joint (see 2.7.) were measured in normal animals and in arthriticanimals (5–6 h postinduction).

Palazzo, E., Fu, Y., Ji, G., Maione, S., & Neugebauer, V. (2008). Group III mGluR7 and mGluR8 in the amygdala differentially modulate nocifensive and affective pain behaviors. Neuropharmacology, 55(4), 537-545.

Figure 5 Analysis of mGluR2/3 and mGluR7 surface vs. intracellular distribution in rat brain synaptosomes.

Representative immunoblots showing surface vs. intracellular distribution of mGluR2/3 and mGluR7 receptors (as well as marker proteins: calnexin, ERK1/2 and RasD1/AGS1) in synaptosomal fraction prepared from the dSTR.

Schwendt, M., Reichel, C. M., & See, R. E. (2012). Extinction-dependent alterations in corticostriatal mGluR2/3 and mGluR7 receptors following chronic methamphetamine self-administration in rats. PLoS One, 7(3).