Rabbit Anti-MGMT Recombinant Antibody (VS13-YC728)
CAT#: VS13-YC728
The Rabbit Anti-MGMT Recombinant Antibody is specific for human MGMT. MGMT encodes a DNA repair enzyme for alkylating agent defense, repairing methylated DNA lesions, with promoter methylation associated with cancers like glioblastoma. The anti-MGMT antibody can be used for Western blot (1/1000-1/2000), Immunohistochemistry-paraffin (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32135/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32136/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69881/157873_A_5_2_selected.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69881/157873_A_6_6.jpg
Normal Tissue
Figure 5 Fallopian tube
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69881/157873_A_7_7.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69881/157873_A_7_8.jpg
Normal Tissue
Figure 7 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69881/157873_B_9_5.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69881/157873_A_7_3.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69881/157873_A_9_5.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170430-MGMT
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Specifications
- Immunogen
- Recombinant human MGMT, aa 1-140.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MGMT
- Species Reactivity
- Human
- Applications
- WB, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- O-6-Methylguanine-DNA Methyltransferase; Methylated-DNA--Protein-Cysteine Methyltransferase; -O-Methylguanine-DNA Methyltransferase; O-6-Methylguanine-DNA-Alkyltransferase; EC 2.1.1.63; O6-Methylguanine-DNA Methyltransferase; Methylguanine-DNA Methyltransferase;
- Gene ID
- 4255
- UniProt ID
- P16455
- Long Name
- O-6-Methylguanine-DNA Methyltransferase
- Sequence Similarities
- Belongs to the MGMT family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_002403.2
- Function
- Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-2228 | Hi-Affi™ Recombinant Rabbit Anti-MGMT Monoclonal Antibody (DS2228AB) | IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2032-CN | Rabbit Anti-MGMT Polyclonal Antibody (MRO-2032-CN) | WB, IF, IHC, FC | Rabbit IgG |
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| VS3-CJ703 | Rabbit Anti-MGMT Recombinant Antibody (VS3-CJ703) | WB, IHC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC140 | AbPlus™ Anti-MGMT Magnetic Beads (VS-0724-YC140) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1346 | Anti-MGMT Immunohistochemistry Kit | IHC | |
| VS-0525-XY4383 | Anti-Human MGMT Immunohistochemistry Kit | IHC |
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