Mouse Anti-MHV Recombinant Antibody (clone 7-10A) (CAT#: FAMAB-0226CQ)

Figure 1 ELISA of mAb binding to immobilized phage (A) and to immobilized MHV-A59 (B) under reducing and nonreducing conditions.

Results are representative of at least two separate experiments.

Mathilde, W. N., Scott, J. K., Fournier, A., & Talbot, P. J. (2000). Characterization of murine coronavirus neutralization epitopes with phage-displayed peptides. Virology, 271(1), 182-196.

Figure 2 Survival of BALB/c and C57BL/6 mice immunized with phage-displayed mAb-selected peptides.

Groups of six mice were immunized ip with different selected phage and then challenged intracerebrally with 10 LD50 of MHV-A59. Phage clones selected by mAb 7-10A: 10.3 (■), 11.1 (●), 11.5 (▲), 12.4 (♦), 12.9 (▼). Clones selected by mAb 5B19: 33.2 (■). Clones selected by mAb 5B170: 9.1 (■), 7.2 (●), 8.3 (▼), 8.1 (♦). Control vector phage, f88.4 (▲). Protection of C57BL/6 mice by clone 9.1 was statistically significant and is representative of two separate experiments.

Mathilde, W. N., Scott, J. K., Fournier, A., & Talbot, P. J. (2000). Characterization of murine coronavirus neutralization epitopes with phage-displayed peptides. Virology, 271(1), 182-196.

Figure 3 Biological half-life of scFv 7-10A.

Two BALB/c mice received a 15-pg injection of purified radioiodinated scFv fragments. At the times indicated, small plasma samples were obtained and circulating levels of scFv fragments were quantitated by densitometric analysis (panel A) of autoradiograms of SDS-PAGE gels (panel B). The estimated half-life was about 6 min.

Lamarre, A., Yu, M. W., Chagnon, F., & Talbot, P. J. (1997). A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice. European journal of immunology, 27(12), 3447-3455.

Figure 4 Reactivity of the scFv fragments for MHV antigen.

Microtiter plates were coated with 500 ng/well of viral antigen preparations (■) or uninfected cell lysates (□). The binding of threefold dilutions of purified scFv fragments was detected using 7-10A-specific anti-idiotype antibodies and horseradish peroxidase-labeled goat anti-rabbit IgG antibodies.

Lamarre, A., Yu, M. W., Chagnon, F., & Talbot, P. J. (1997). A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice. European journal of immunology, 27(12), 3447-3455.

Figure 5 Detection of Id on MHV-A59-specific mAb.

Microtiter plates were coated with 65 ng/well of Abl. control. or MHV-A59-specific mAb and binding of varying amounts of purified Ab2 was determined using horseradish peroxidase-labeled anti-rabbit antibodies.

Lamarre, A., Lecomte, J., & Talbot, P. J. (1991). Antiidiotypic vaccination against murine coronavirus infection. The Journal of Immunology, 147(12), 4256-4262.

Figure 6 Detection of Id in syngeneic (BALB/c mlce). allogeneic (AKR. SJL. and C57BL/6 mice) and xenogeneic (rat and rabbit] antiviral serum.

Microtiter plates were coated with 15 ng/well of purified anti-Id (Ab2) or NRlg. The binding of dilutions of mouse or rat hyperimmune sera produced against MHV-A59. mAb 7-10A (Abl). or biotinylated purified rabbit antiviral Ig was determined using peroxidase-labeled species-specific anti-lg or streptavidin.

Lamarre, A., Lecomte, J., & Talbot, P. J. (1991). Antiidiotypic vaccination against murine coronavirus infection. The Journal of Immunology, 147(12), 4256-4262.