Rabbit Anti-MKI67 Recombinant Antibody (clone AFY0025)
CAT#: MOR-0054-FY
This product is a recombinant Rabbit antibody that recognizes MKI67. The monoclonal Rabbit antibody AFY0025 reacts with the epitope (c-terminal) of MKI67.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/952_H5_3_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/30_B1_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_6_6.jpg
Normal Tissue
Figure 6 Skin
Cells in basal layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Cells in spinous layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Lymphocytes
Staining: High
Intensity: Strong
Sebaceous glands
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_B_7_1.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
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Specifications
- Immunogen
- C-terminal half of human Proliferation marker protein Ki-67.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MKI67
- Species Reactivity
- Human
- Clone
- AFY0025
- Applications
- ICC, IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Lyophilized
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 5% Trehalose
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "MKI67"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1072z | Mouse Anti-MKI67 Recombinant Antibody (clone 26F12) | WB, ELISA, IHC | Mouse IgG1 |
| ZG-0403U | Mouse Anti-MKI67 Recombinant Antibody (clone M25-Y) | IHC-P | Mouse IgG |
| VS3-CJ971 | Mouse Anti-MKI67 Recombinant Antibody (clone ABT-KI67) | IF, IHC-P | Mouse IgG1, κ |
| VS3-CJ972 | Mouse Anti-MKI67 Recombinant Antibody (clone PT2017) | IF, IHC-P | Mouse IgG |
| VS3-CJ973 | Rabbit Anti-MKI67 Recombinant Antibody (VS3-CJ973) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2237 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (DS2237AB) | ELISA, WB, IHC | IgG |
| MOR-4638 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (TH151DS) | IF, ICC, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2357LY-F(E) | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); Fab Fragment | IHC, ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2357LY-S(P) | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); scfv Fragment | IHC, ELISA, WB | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC730 | CytoStream™ Rabbit Anti-MKI67 Recombinant Antibody (VS13-YC730) | IHC-P, IHC-Fr, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4403 | Anti-MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4404 | Anti-Mouse MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4405 | Anti-Rabbit MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4406 | Anti-Canine MKI67 Immunohistochemistry Kit | IHC |
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