CytoStream™ Rabbit Anti-MLH1 Recombinant Antibody (VS13-YC731)
CAT#: VS13-YC731
The CytoStream™ Rabbit Anti-MLH1 Recombinant Antibody is specific for human, mouse and rat MLH1. MLH1, encoding a protein for DNA mismatch repair as part of MutL alpha complexes, relates to hereditary nonpolyposis colon cancer, also involved in DNA damage signaling. The anti-MLH1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60714/1149_G9_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13294/ihc_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_4_6.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_9_3.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076242-MLH1
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Specifications
- Immunogen
- Recombinant human MLH1, aa 707-756.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MLH1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- MutL Homolog 1; COCA2; MutL (E. Coli) Homolog 1 (Colon Cancer, Nonpolyposis Type 2); MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli); MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2; DNA Mismatch Repair Protein Mlh1;
- Gene ID
- 4292
- UniProt ID
- P40692
- Long Name
- MutL Homolog 1
- Sequence Similarities
- Belongs to the DNA mismatch repair MutL/HexB family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_000240.1; NP_001161089.1; NP_001161090.1
- Function
- Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MLH1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2242 | Hi-Affi™ Recombinant Rabbit Anti-MLH1 Monoclonal Antibody (DS2242AB) | WB, IP, IHC-P, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY931 | Recombinant Mouse Anti-MLH1 Antibody (clone 2B1C5) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY932 | Recombinant Mouse Anti-MLH1 Antibody (clone 5A3F6) | ELISA, FC | Mouse IgG2b |
| VS3-FY933 | Recombinant Mouse Anti-MLH1 Antibody (clone 7A7C8) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY934 | Recombinant Mouse Anti-MLH1 Antibody (clone 8C12B7) | ELISA, ICC, FC | Mouse IgG2b |
| VS3-FY935 | Recombinant Rabbit Anti-MLH1 Antibody (clone R02-2E8) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY324 | Mouse Anti-NHP MLH1 Recombinant Antibody (clone 4C9C7) | WB, IHC, ICC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4417 | Anti-MLH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4418 | Anti-Rat MLH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4419 | Anti-Mouse MLH1 Immunohistochemistry Kit | IHC |
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