Rabbit Anti-MMP9 Recombinant Antibody (clone 29C11)

Name: Rabbit Anti-MMP9 Recombinant Antibody (clone 29C11)
SKU: ZG-0471U
Rating: 5 (1 reviews)

CAT#: ZG-0471U

Rabbit Anti-MMP9 Antibody (clone 29C11) displayed high specificity against the MMP9 protein of Human.

Tested Data Gene Expression

IHC

Figure 1 Rabbit Anti-MMP9 Antibody (ZG-0471U) in IHC

IHC image of ZG-0471U diluted at 1:235 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 2 Rabbit Anti-MMP9 Antibody (ZG-0471U) in FC

Overlay histogram showing Jurkat cells stained with ZG-0471U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10, 000 events was performed.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg

Subcellular Location Figure 2 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg

Normal Tissue Figure 5 Testis

Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enriched (NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9

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Specifications

Immunogen
A synthesized peptide derived from human MMP9

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human MMP9

Species Reactivity
Human

Clone
29C11

Applications
ELISA, IHC, FC

Conjugate
Unconjugated

Related Disease
Metaphyseal Anadysplasia 2 and Metaphyseal Anadysplasia.

Product Property

Purification
Affinity chromatography purified

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry. Specific conditions should be optimized by the end user.
IHC:1:50-1:200

Target

Alternative Names
Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; MMP9; CLG4B

Gene ID
4318

UniProt ID
P14780

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Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0471U.pdf

MSDS

MSDS - ZG-0471U.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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