CytoStream™ Rabbit Anti-MMP9 Recombinant Antibody (VS13-YC735)
CAT#: VS13-YC735
The CytoStream™ Rabbit Anti-MMP9 Recombinant Antibody is specific for human and rat MMP9. MMP9 belongs to the metalloproteinase family, cleaving extracellular matrix components in physiological and pathological processes, associated with progenitor cell mobilization and tissue remodeling. The anti-MMP9 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
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Specifications
- Immunogen
- Recombinant human MMP9, aa 71-120.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat MMP9
- Species Reactivity
- Human, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Metaphyseal Anadysplasia; Metaphyseal Anadysplasia
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Matrix Metallopeptidase 9; Matrix Metalloproteinase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase); EC 3.4.24.35; CLG4B; MMP-9; GELB; Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase); Matrix Metalloproteinase-9;
- Gene ID
- 4318
- UniProt ID
- P14780
- Long Name
- Matrix Metallopeptidase 9
- Cellular Localization
- Secreted
- Protein Refseq
- NP_004985.2
- Function
- Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, 1480034, 12879005).
Could play a role in bone osteoclastic resorption (By similarity).
Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005).
Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094).
Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034).
Degrades fibronectin but not laminin or Pz-peptide.
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MMP9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1048 | Recombinant Mouse Anti-Human MMP9 Antibody Fab Fragment | LMNX, ELISA, WB, IF | Fab |
| HPAB-0027-YC-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (HPAB-0027-YC-F(E)) | FC | Mouse Fab |
| HPAB-0028-YC-F(E) | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1); Fab Fragment | Neut | Human Fab |
| HPAB-0513LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); Fab Fragment | FC | Mouse Fab |
| HPAB-0514LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); Fab Fragment | FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-533CL | Anti-Human MMP9 Recombinant Antibody (TAB-533CL) | WB, Inhib | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0824CL | Human Anti-MMP9 Recombinant Antibody (TAB-0824CL) | ELISA | Human IgG4 |
| TAB-0825CL | Human Anti-MMP9 Recombinant Antibody (TAB-0825CL) | ELISA | Human IgG4 |
| TAB-0824CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0824CL-S(P)) | ELISA | Human scFv |
| TAB-0825CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0825CL-S(P)) | ELISA | Human scFv |
| TAB-0824CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0824CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0826CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0826CL) | Inhib, ELISA, IHC | Mouse IgG |
| TAB-0827CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0827CL) | ELISA | Mouse IgG |
| TAB-0826CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0826CL-S(P)) | ELISA | Mouse scFv |
| TAB-0827CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0827CL-S(P)) | ELISA | Mouse scFv |
| TAB-0826CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0826CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0717MZ | Chicken Anti-MMP9 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1748CQ | Recombinant Mouse Anti-MMP9 Antibody (VMC204) | Inhib, IHC-Fr, IP, IHC-P, WB, IHC, ICC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2263 | Hi-Affi™ Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (DS2263AB) | FC, IHC-P, WB | IgG |
| MOR-4331 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI420DS) | ELISA | IgG |
| MOR-4332 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI421DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002ML | Anti-Human MMP9 Recombinant Antibody (TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0200-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0513LY-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); scfv Fragment | FC | Mouse scfv |
| HPAB-0514LY-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); scfv Fragment | FC | Mouse scfv |
| HPAB-1320-FY-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (HPAB-1320-FY-F(E)) | Activ | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1014-CN | Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (JA80-73) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0706F | Mouse Anti-MMP9 Recombinant Antibody (clone 2C3) | WB, IHC-P | Mouse IgG1 |
| ZG-0728F | Mouse Anti-MMP9 Recombinant Antibody (clone 4A3) | WB, IHC-P | Mouse IgG1 |
| VS3-WK1740 | Recombinant Rabbit Anti-MMP9 Capture Antibody (clone R04-8Z7) | ELISA | Rabbit IgG |
| VS3-WK1741 | Recombinant Rabbit Anti-MMP9 Detection Antibody (clone R07-8E3) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-002ML | Afuco™ Anti-MMP9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX783 | Mouse Anti-MMP9 Recombinant Antibody (clone 331CT17.4.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY101 | Human Anti-MMP9 (clone DX-2802) scFv-Fc Chimera | WB, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1357 | Anti-MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4460 | Anti-Mouse MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4461 | Anti-Zebrafish MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4459 | Anti-Human MMP9 Immunohistochemistry Kit | IHC |
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