Anti-Mouse AFAP1 Immunohistochemistry Kit (CAT#: VS-0525-XY202)
This AFAP1 IHC kit offers a complete reagent set for effective and accurate immunohistochemical staining. It is optimized for use with both paraffin-embedded and frozen tissue sections, showing broad reactivity across multiple species. The easy-to-follow protocol ensures reproducible and high-quality results for AFAP1 detection.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol, actin filaments & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15642/165_G10_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol, actin filaments & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15642/165_G10_2_selected.jpg

Figure 2 Testis
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_4_6.jpg
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_4_6.jpg

Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_7_8.jpg

Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_B_9_5.jpg

Figure 5 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_9_3.jpg
(Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_9_3.jpg

Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_8_4.jpg

Figure 7 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24712/63659_A_9_5.jpg

Figure 8 RNA cell line category: Cell line enhanced (TIME)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196526-AFAP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196526-AFAP1
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- AFAP1
- Primary Antibody
- Mouse Anti-AFAP1 Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3893z | Mouse Anti-AFAP1 Recombinant Antibody (clone 25A2) | WB, IHC | Mouse IgG1 |
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