Anti-Mouse EED Immunohistochemistry Kit
CAT#: VS-0525-XY2160
The EED IHC kit offers complete reagents for detecting this chromatin-modifying protein, part of the polycomb repressive complex. It works on paraffin and frozen sections from various species, supporting research on gene silencing, developmental biology, and cancer epigenetics.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60089/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61140/138483_A_8_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in lymphoid cells outside reaction centra.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12211/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60089/1259_G9_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60089/1006_G12_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61140/138483_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61140/138483_A_8_3.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61140/138483_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61140/138483_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074266-EED
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat, Bovine, Chicken, Frog, Zebrafish
- Target
- EED
- Primary Antibody
- Mouse Anti-EED Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EED"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-313 | Human Anti-EED Recombinant Antibody (clone D424) | ELISA, IP-MS | Human IgG |
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