Anti-Mouse SMAD4 Immunohistochemistry Kit
CAT#: VS-0525-XY6721
The SMAD4 IHC Kit provides ready-to-use reagents for detecting SMAD4 in various tissues. It works on paraffin-embedded and frozen sections, supporting cross-species studies on tumor suppression, signaling pathways, and cell cycle regulation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_6_6.jpg
Normal Tissue
Figure 8 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_2_7.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141646-SMAD4
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- SMAD4
- Primary Antibody
- Rabbit Anti-SMAD4 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Diseases
Colorectal Cancer
Gastric Cancer
Pancreatic Cancer
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SMAD4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2804z | Mouse Anti-SMAD4 Recombinant Antibody (clone 22G1) | WB, ELISA, FC, IF, IHC | Mouse IgG |
| MRO-1405-CN | Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (CBACN-513) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2194-CN | Rabbit Anti-SMAD4 Polyclonal Antibody (MRO-2194-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-QX1028 | Mouse Anti-SMAD4 Recombinant Antibody (clone 5B8-F7-F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1359 | Recombinant Rabbit Anti-SMAD4 Antibody (clone R05-1I7) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3323 | Hi-Affi™ Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (DS3323AB) | WB, IHC-P, ICC, FC, IP | IgG |
| MOR-4736 | Hi-Affi™ Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (TH250DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC832 | AbPlus™ Anti-SMAD4 Magnetic Beads (VS-0724-YC832) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY201 | Human Anti-SMAD4 scFv-Fc Chimera (VS-0325-FY201) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2107 | Anti-SMAD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6720 | Anti-Human SMAD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6722 | Anti-Rat SMAD4 Immunohistochemistry Kit | IHC |
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