Anti-MPL Antibody Prodrug, Protease Activated (VS-1025-YC27)

Name: Anti-MPL Antibody Prodrug, Protease Activated (VS-1025-YC27)
SKU: VS-1025-YC27
Rating: 5 (1 reviews)

CAT#: VS-1025-YC27

The Anti-MPL Antibody Prodrug, Protease Activated (VS-1025-YC27) is an antibody prodrug comprised of a recombinant mouse antibody targeting human MPL, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to MPL protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to MPL effectively. MPL (Myeloproliferative Leukemia virus oncogene), or the Thrombopoietin Receptor, is often implicated in myeloproliferative neoplasms (MPNs). A common point mutation (W515L/K) in its cytoplasmic domain causes constitutive activation of the JAK/STAT signaling pathway, leading to the overproduction of blood cells. This dysregulation is central to the pathogenesis of primary myelofibrosis and essential thrombocythemia.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to nuclear membrane & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7619/1825_E1_28_cr5abb7d728bca3_selected.jpg

RNA Expression Figure 2 RNA cell line category: Cell line enriched (HEL)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117400-MPL

❮

❯

Specifications

Host Animal
Mouse

Applications
In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay

Specificity
Human MPL

Target
MPL

Species Reactivity
Human

Isotype
IgG2b, kappa

Epitope
MPL-ECD

Masking Approach
Affinity mask

Mask
A synthetic peptide

Protease Cleavable Linker
MMP-cleavable linker

Protease Substrate
MMPs

Purification
Protein A purified

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target

Introduction
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

Alternative Names
MPLV; TPOR; C-MPL; CD110; THCYT2

Gene ID
4352

UniProt ID
P40238

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for VS-1025-YC27. Click the button above to contact us or submit your feedback about this product.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Datasheet

Datasheet - VS-1025-YC27.pdf

MSDS

MSDS - VS-1025-YC27.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "MPL"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-2377z	Mouse Anti-MPL Recombinant Antibody (clone 42A11)	WB, ELISA, FC, ICC, IF, IHC	Mouse IgG1

CAT	Product Name	Application	Type
TAB-155CQ	Human Anti-MPL Recombinant Antibody (TAB-155CQ)	ELISA, FC, FuncS	Human IgG1

CAT	Product Name	Application	Type
TAB-156CQ	Anti-Human MPL Recombinant Antibody (TA136)	ELISA, FC

CAT	Product Name	Application	Type
TAB-157CQ	Anti-Human MPL Recombinant Antibody (VB22B)	ELISA, FC

CAT	Product Name	Application	Type
TAB-155CQ-S(P)	Human Anti-MPL Recombinant Antibody; scFv Fragment (TAB-155CQ-S(P))	ELISA, FC, WB	Human scFv

CAT	Product Name	Application	Type
TAB-156CQ-S(P)	Anti-Human MPL Recombinant Antibody scFv Fragment (TA136)	ELISA, FC

CAT	Product Name	Application	Type
TAB-157CQ-S(P)	Anti-Human MPL Recombinant Antibody scFv Fragment (VB22B)	ELISA, FC

CAT	Product Name	Application	Type
TAB-155CQ-F(E)	Human Anti-MPL Recombinant Antibody; Fab Fragment (TAB-155CQ-F(E))	ELISA, FC, WB	Human Fab

CAT	Product Name	Application	Type
TAB-156CQ-F(E)	Anti-Human MPL Recombinant Antibody Fab Fragment (TA136)	ELISA, FC

CAT	Product Name	Application	Type
MOR-2272	Hi-Affi™ Recombinant Rabbit Anti-MPL Monoclonal Antibody (DS2272AB)	FC, WB	IgG

CAT	Product Name	Application	Type
MOB-108LC	Recombinant Human Anti-Human MPL Agonist Antibody (12B5)	FC, FunS, ELISA	Human antibody

CAT	Product Name	Application	Type
MOB-109LC	Recombinant Human Anti-Human MPL Agonist Antibody (12D5)	FC, FunS, ELISA	Human antibody

CAT	Product Name	Application	Type
MOB-110LC	Recombinant Human Anti-Human MPL Agonist Antibody (12E10)	FC, FunS, ELISA	Human antibody

CAT	Product Name	Application	Type
HPAB-0566-CN	Mouse Anti-MPL Recombinant Antibody (HPAB-0566-CN)	ELISA, FC, Block	Mouse IgG2b, κ

CAT	Product Name	Application	Type
HPAB-0567-CN	Mouse Anti-MPL Recombinant Antibody (HPAB-0567-CN)	ELISA, FC, Block	Mouse IgG2b, κ

CAT	Product Name	Application	Type
HPAB-0568-CN	Mouse Anti-MPL Recombinant Antibody (HPAB-0568-CN)	ELISA, FC, Block	Mouse IgG1, κ

CAT	Product Name	Application	Type
HPAB-0566-CN-S(P)	Mouse Anti-MPL Recombinant Antibody; scFv Fragment (HPAB-0566-CN-S(P))	ELISA, FC, Block	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0567-CN-S(P)	Mouse Anti-MPL Recombinant Antibody; scFv Fragment (HPAB-0567-CN-S(P))	ELISA, FC, Block	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0568-CN-S(P)	Mouse Anti-MPL Recombinant Antibody; scFv Fragment (HPAB-0568-CN-S(P))	ELISA, FC, Block	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0566-CN-F(E)	Mouse Anti-MPL Recombinant Antibody; Fab Fragment (HPAB-0566-CN-F(E))	ELISA, FC, Block	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0567-CN-F(E)	Mouse Anti-MPL Recombinant Antibody; Fab Fragment (HPAB-0567-CN-F(E))	ELISA, FC, Block	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0568-CN-F(E)	Mouse Anti-MPL Recombinant Antibody; Fab Fragment (HPAB-0568-CN-F(E))	ELISA, FC, Block	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0411-YC	Mouse Anti-MPL Recombinant Antibody (HPAB-0411-YC)	FC, ELISA	Mouse IgG1, κ

CAT	Product Name	Application	Type
HPAB-0411-YC-S(P)	Mouse Anti-MPL Recombinant Antibody; scFv Fragment (HPAB-0411-YC-S(P))	FC, ELISA	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0412-YC-S(P)	Human Anti-MPL Recombinant Antibody scFv Fragment (HPAB-0412-YC-S(P))	FC, FuncS	Human scFv

CAT	Product Name	Application	Type
HPAB-0411-YC-F(E)	Mouse Anti-MPL Recombinant Antibody; Fab Fragment (HPAB-0411-YC-F(E))	FC, ELISA	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0412-YC-F(E)	Human Anti-MPL Recombinant Antibody Fab Fragment (HPAB-0412-YC-F(E))	FC, FuncS	Human Fab

CAT	Product Name	Application	Type
VS-0325-FY143	Human Anti-MPL (clone 5E5) scFv-Fc Chimera	ELISA	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC552	Recombinant Anti-MPL Vesicular Antibody, EV Displayed (VS-0425-YC552)	Block, ELISA, FC, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-YC127	Recombinant Anti-MPL (Bin I x Bin II) Biparatopic Antibody, Tandem scFv	Block, ELISA, FC	Tandem scFv

Specific Inquiry

Add to compare

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Anti-Human CD22 Recombinant Antibody (TAB-183-F(E)) in HPLC

Anti-Human CD22 Recombinant Antibody (TAB-183-F(E)) (CAT# TAB-183-F(E))
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC

Figure 2 Anti-Human CXCR4 Recombinant Antibody (TAB-H72) in HPLC

Human Anti-Human CXCR4 Recombinant Antibody (TAB-H72) (CAT# TAB-H72)
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC

Figure 1 Anti-Human ERBB3 Recombinant Antibody (TAB-H47) in SDS-PAGE

Humanized Anti-Human ERBB3 Recombinant Antibody (TAB-H47) (CAT# TAB-H47)
Application: ELISA, Inhib

Figure 2 Anti-Human CD23 Recombinant Antibody (Gomiliximab) (TAB-231) in ELISA

Anti-Human CD23 Recombinant Antibody (Gomiliximab) (CAT# TAB-231)
Application: WB, Neut, ELISA, IF, IP, FuncS, FC

Figure 2 Anti-Influenza Virus M2e Antibody (TAB-508CL) in HPLC

Anti-Influenza Virus M2e Recombinant Antibody (TCN-032) (CAT# TAB-508CL)
Application: FuncS

Figure 11 Anti-IAV HA Recombinant Antibody (PABL-214) in DB

Recombinant Human Anti-IAV HA Antibody (FI6V3) (CAT# PABL-214)
Application: WB, IHC, FuncS

Figure 2 Anti-C3b Antibody (PABL-420) in HPLC

Human Anti-C3b Recombinant Antibody (clone S77) (CAT# PABL-420)
Application: FuncS, Inhib, IP, ELISA

Figure 5 Recombinant Mouse Anti-DENV-3 Antibody (PABL-446) in HPLC

Recombinant Mouse Anti-DENV-3 Antibody (mAb 4E11) (CAT# PABL-446)
Application: WB, FuncS

Figure 1 Anti-B. anthracis protective antigen (PA) Antibody (MRO-073CT) in SDS-PAGE

Anti-B. anthracis protective antigen (PA) Recombinant Antibody (14B7) (CAT# MRO-073CT)
Application: Neut, FC

Figure 4 Anti-Influenza A Virus HA Recombinant Antibody (MRO-248LC) in DB

Anti-Influenza A Virus (H1N1/H2N2) HA Recombinant Antibody (C179) (CAT# MRO-248LC)
Application: ELISA

Figure 5 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in SEC-HPLC.

Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (CAT# MRO-961CQ)
Application: ELISA

Figure 3 Anti-Human ENG Antibody (TAB-013ML) in DB

Anti-Human ENG Recombinant Antibody (TAB-013ML) (CAT# TAB-013ML)
Application: ELISA, IHC, FC, IP, IF, Inhib

Figure 3 Anti-Human CD274 Antibody (TAB-417CQ) in DB

Anti-Human CD274 Recombinant Antibody (TAB-417CQ) (CAT# TAB-417CQ)
Application: ELISA, IHC, FC, IP, IF, Inhib

Figure 3 Mouse Anti-CEACAM6 Antibody (HPAB-0286-CN) in DB

Human Anti-CEACAM6 Recombinant Antibody (HPAB-0286-CN) (CAT# HPAB-0286-CN)
Application: ELISA, WB, FC

Figure 2 Mouse Anti-Cs Recombinant Antibody (FAMAB-0077YC) in ELISA

Mouse Anti-Cs Recombinant Antibody (clone R45-45-11) (CAT# FAMAB-0077YC)
Application: ELISA

Figure 2 Mouse Anti-C5 Recombinant Antibody (HPAB-0128CQ) in ELISA

Mouse Anti-C5 Recombinant Antibody (clone BB5.1) (CAT# HPAB-0128CQ)
Application: FC, IA, IF, IP, IHC, FuncS

Figure 5 Anti-Human CDH17 Recombinant Antibody (HPAB-2061LY) in ELISA

Human Anti-CDH17 Recombinant Antibody (clone D2101) (CAT# HPAB-2061LY)
Application: ELISA

Figure 6 Mouse Anti-Citrullinated epitope Recombinant Antibody, mouse IgG2a (HPAB-AP605-YC-mG2a) in DB

Mouse Anti-Citrullinated epitope Recombinant Antibody (HPAB-AP605-YC) (CAT# HPAB-AP605-YC)
Application: ELISA, IHC, WB

Figure 5 Human Anti-IAV H3N2 HA Recombinant Antibody (PABJ-0152) in Dot Blot

Human Anti-IAV H3N2 HA Recombinant Antibody (clone S5V2-29) (CAT# PABJ-0152)
Application: ELISA, Neut, FuncS

Figure 17 Human Anti-IAV HA Recombinant Antibody (PABJ-0204) in DB

Human Anti-IAV HA Recombinant Antibody (PABJ-0204) (CAT# PABJ-0204)
Application: ELISA, Inhib, FuncS

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.