Rabbit Anti-MRPL28 Recombinant Antibody (clone R62-1L-4)
CAT#: VS3-FY2136
This R62-1L-4 recombinant monoclonal antibody is developed to recognize human MRPL28 protein, a calculated weight of 30 kDa. It cross-reacts with mouse, rat. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30594/if_selected.jpg
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MRPL28
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R62-1L-4
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 30 kDa, while the observed molecular weight stands at 30kDa.
- Related Disease
- Pancreatic Serous Cystadenoma and Megalencephalic Leukoencephalopathy With Subcortical Cysts 1
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/50
Flow Cytometry: 1/50
Target
- Alternative Names
- Mitochondrial Ribosomal Protein L28; Melanoma-Associated Antigen Recognised By Cytotoxic T Lymphocytes; Melanoma-Associated Antigen Recognized By T-Lymphocytes; Mitochondrial Large Ribosomal Subunit Protein BL28m; Melanoma Antigen P15; MRP-L28
- Gene ID
- 10573
- UniProt ID
- Q13084
- Sequence Similarities
- Belongs to the bacterial ribosomal protein bL28 family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Ubiquitination at Lys106 (NX_Q13084 [NX_Q13084-1])
Modification sites at PhosphoSitePlus (Q13084)
Glycosylation from GlyGen (Q13084) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005255098.1; XP_011520653.1; XP_054235367.1; NP_006419.2
- Function
- Potentially represents an important therapeutic reagent for HLA-A24 patients. This antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290 in the context of HLA-A24.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MRPL28"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1817z | Mouse Anti-MRPL28 Recombinant Antibody (clone 32F2) | WB, ELISA, IF, IP | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0375MZ | Chicken Anti-MRPL28 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2286 | Hi-Affi™ Recombinant Rabbit Anti-MRPL28 Monoclonal Antibody (DS2286AB) | WB, ICC, IP, FC | IgG |
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