Rabbit Anti-MRPL28 Recombinant Antibody (clone R62-1L-4) (CAT#: VS3-FY2136)
This R62-1L-4 recombinant monoclonal antibody is developed to recognize human MRPL28 protein, a calculated weight of 30 kDa. It cross-reacts with mouse, rat. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30594/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30594/if_selected.jpg
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30594/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30594/ihc_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55589/140454_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55589/140454_A_8_8.jpg
Figure 4 Gallbladder
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55589/140454_A_5_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55589/140454_A_5_4.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000086504-MRPL28
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000086504-MRPL28
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MRPL28
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R62-1L-4
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 30 kDa, while the observed molecular weight stands at 30kDa.
- Related Disease
- Pancreatic Serous Cystadenoma and Megalencephalic Leukoencephalopathy With Subcortical Cysts 1
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/50
Flow Cytometry: 1/50
Target
- Alternative Names
- Mitochondrial Ribosomal Protein L28; Melanoma-Associated Antigen Recognised By Cytotoxic T Lymphocytes; Melanoma-Associated Antigen Recognized By T-Lymphocytes; Mitochondrial Large Ribosomal Subunit Protein BL28m; Melanoma Antigen P15; MRP-L28
- Gene ID
- 10573
- UniProt ID
- Q13084
- Sequence Similarities
- Belongs to the bacterial ribosomal protein bL28 family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Ubiquitination at Lys106 (NX_Q13084 [NX_Q13084-1])
Modification sites at PhosphoSitePlus (Q13084)
Glycosylation from GlyGen (Q13084) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005255098.1; XP_011520653.1; XP_054235367.1; NP_006419.2
- Function
- Potentially represents an important therapeutic reagent for HLA-A24 patients. This antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290 in the context of HLA-A24.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1817z | Mouse Anti-MRPL28 Recombinant Antibody (clone 32F2) | WB, ELISA, IF, IP | Mouse IgM, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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