Rabbit Anti-MRPS18B Recombinant Antibody (clone R05-0C8)
CAT#: VS3-FY2039
The R05-0C8 recombinant monoclonal antibody was developed to recognize human MRPS18B. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. The specificity of the MRPS18B antibody was confirmed by western blot analysis of MRPS18B in Raw264.7 lysates and western blot analysis of MRPS18B in A549, HL-60, U251 lysates.
Tested Data
WB
Figure 1 Anti-MRPS18B Antibody (VS3-FY2039) in WB.
The MRPS18B expression in Raw264.7 lysates were detected using the anti-MRPS18B by western blot assay.
WB
Figure 2 Anti-MRPS18B Antibody (VS3-FY2039) in WB.
The MRPS18B expression in A549, HL-60, U251 lysates were detected using the anti-MRPS18B by western blot assay.
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Specifications
- Immunogen
- Recombinant human MRPS18B protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MRPS18B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-0C8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 29 kDa, while the observed molecular weight stands at 29 kDa.
- Related Disease
- Loeys-Dietz Syndrome 4 and Megaesophagus
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitochondrial Ribosomal Protein S18B; Mitochondrial Small Ribosomal Subunit Protein BS18b; Mitochondrial Small Ribosomal Subunit Protein MS40; 28S Ribosomal Protein S18-2, Mitochondrial; MRP-S18-2; MRP-S18-B; Mrps18-B; C6orf14; S18mt-B
- Gene ID
- 28973
- UniProt ID
- Q9Y676
- Sequence Similarities
- Belongs to the bacterial ribosomal protein bS18 family. Mitochondrion-specific ribosomal protein mS40 subfamily.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9Y676)
Modification sites at neXtProt (NX_Q9Y676)
Glycosylation from GlyGen (Q9Y676) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_024302176.2; XP_054185773.1; XP_054186261.1; XP_054186552.1; XP_054186778.1
- Function
- There are 3 mitochondrial isoforms of bS18 in mammalia, localizing to 3 distinct sites in the mitoribosome. bS18m (bs18c) binds to the same site as bacterial bS18, mS40 (bS18b, this protein) binds to a novel location of the 28S small subunit, and mL66 (bS18a) binds to the 39S large subunit.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
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