Mouse Anti-MSH6 Recombinant Antibody (clone 1D10G1)
CAT#: VS7-0425-WR420
This product is a mouse IgG1 antibody that binds to human MSH6. The antibody 1D10G1 recognizes specific amino acid domain of human MSH6 (AA: 217-395). It can be used for WB and ELISA assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9091/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, golgi apparatus & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/290_E8_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/249_E8_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_8_5.jpg
Normal Tissue
Figure 10 Testis
Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_6_6.jpg
Normal Tissue
Figure 11 Ovary
Ovarian stroma cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_4_7.jpg
Normal Tissue
Figure 12 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116062-MSH6
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Specifications
- Immunogen
- Recombinant human MSH6 (AA: 217-395) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MSH6
- Species Reactivity
- Human
- Clone
- 1D10G1
- Molecule Class
- Dna Mismatch Repair Protein
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Lynch syndrome 5 (LYNCH5); Endometrial cancer (ENDMC); Mismatch repair cancer syndrome 3 (MMRCS3); Colorectal cancer (CRC)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MSH6; mutS homolog 6; GTBP; HSAP; p160; GTMBP; HNPCC5; DNA mismatch repair protein Msh6; G/T mismatch-binding protein; mutS-alpha 160 kDa subunit; sperm-associated protein
- Gene ID
- 2956
- UniProt ID
- P52701
- Long Name
- MutS Homolog 6
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_000170.1
- Function
- DNA-binding
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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