Mouse Anti-MSLN Recombinant Antibody (clone MB) (CAT#: HPAB-1781-FY)
This product is a recombinant antibody that recognizes MSLN. The monoclonal antibody MB reacts with the epitope of MSLN.
Specific Inquiry
ELISA
Figure 1 MAbreactivity on ELISA coated with mesothelin-Fc.
ELISA plates were coated with mesothelin-Fc (2 μg/mL). Then, ELISA plates were incubated with anti-mesothelin MAbs, MN, MB, K1 or 5B2, followed by horseradish peroxidase-conjugated goat anti-mouse IgG.
Onda, M., Willingham, M., Nagata, S., Bera, T. K., Beers, R., Ho, M., ... & Pastan, I. (2005). New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clinical cancer research, 11(16), 5840-5846.
ELISA
Figure 2 MAb reactivity on ELISA coated with bacterial maltose-binding protein-mesothelin.
ELISA plates were coated with bacterial mesothelin (2 μg/mL).Then, ELISA plates were incubated with anti-mesothelin MAbs, MN, MB, K1 or 5B2, followed by horseradish peroxidase-conjugated goat anti-mouse IgG.
Onda, M., Willingham, M., Nagata, S., Bera, T. K., Beers, R., Ho, M., ... & Pastan, I. (2005). New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clinical cancer research, 11(16), 5840-5846.
WB
Figure 3 Western blot analysis of anti-mesothelin MAbs.
Mesothelin-Fc (lane1,100 ng; lane 2, 25 ng; lane 3, 6 ng; lane 4, 2 ng; and lane 5, 0.4 ng) and CD25-Fc (lane 6, 50 ng) were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes. Membranes were probed with each anti-mesothelin MAbs (1 μg/mL) or anti-CD30 MAb,T6 (control), followed byALP-goat anti-mouse IgG and BCIP/nitroblue tetrazolium substrate.
Onda, M., Willingham, M., Nagata, S., Bera, T. K., Beers, R., Ho, M., ... & Pastan, I. (2005). New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clinical cancer reseah, 11(16), 5840-5846.
IHC
Figure 4 Immunohistochemistry of anti-mesothelin MAbs.
Anti-mesothelin primary antibody MB was used to label the section.
Onda, M., Willingham, M., Nagata, S., Bera, T. K., Beers, R., Ho, M., ... & Pastan, I. (2005). New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clinical cancer reseah, 11(16), 5840-5846.
IHC
Figure 5 Immunohistochemistry of anti-mesothelin MAbs on tissue arrays.
Onda, M., Willingham, M., Nagata, S., Bera, T. K., Beers, R., Ho, M., ... & Pastan, I. (2005). New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clinical cancer reseah, 11(16), 5840-5846.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MSLN
- Species Reactivity
- Human
- Clone
- MB
- Applications
- ELISA, WB, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The MSLN antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB: 1 μg/mL.
Target
- Alternative Names
- Mesothelin; Pre-Pro-Megakaryocyte-Potentiating Factor; CAK1 Antigen; MPF; Soluble MPF Mesothelin Related Protein; Megakaryocyte Potentiating Factor; SMRP
- Gene ID
- 10232
- UniProt ID
- Q13421
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone MB"
See other products for "MSLN"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-009 | Recombinant Anti-human MSLN VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
| HPAB-M0391-YC | Recombinant Llama Anti-MSLN Single Domain Antibody (NbA1/G3A) | ELISA, FC, IF | Llama VHH |
| HPAB-M0392-YC | Recombinant Llama Anti-MSLN Single Domain Antibody (NbC6/F10) | ELISA, FC, IF | Llama VHH |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-201 | Anti-Human Mesothelin Recombinant Antibody (Amatuximab) | IP, IF, FuncS, FC, Neut, ELISA, IHC | IgG1 - kappa |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G052E | Anti-MSLN immunotoxin RG7787 (Fab)-PE | Cytotoxicity assay, Function study | |
| AGTO-G053E | Anti-Msln immunotoxin (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-L035E | anti-MSLN immunotoxin K1 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L063E | anti-MSLN immunotoxin 8B35 (scFv)-PE | Cytotoxicity assay, Functional assay |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0833CL | Human Anti-MSLN Recombinant Antibody (TAB-0833CL) | ELISA, FuncS, Activ, FC | Human IgG1 |
| TAB-0834CL | Human Anti-MSLN Recombinant Antibody (TAB-0834CL) | ELISA, FuncS, FC, SPR, Inhib | Human IgG1, κ |
| TAB-0835CL | Human Anti-MSLN Recombinant Antibody (TAB-0835CL) | ELISA, FC, FACS, Inhib | Human IgG1, λ |
| TAB-0833CL-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (TAB-0833CL-S(P)) | ELISA, FC | Human scFv |
| TAB-0834CL-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (TAB-0834CL-S(P)) | ELISA, FC | Human scFv |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0376MZ | Chicken Anti-MSLN Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2302 | Hi-Affi™ Recombinant Rabbit Anti-MSLN Monoclonal Antibody (DS2302AB) | WB | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0041-CN-F(E) | Mouse Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0041-CN-F(E)) | FC, WB, ELISA | Mouse Fab |
| HPAB-0042-CN-F(E) | Mouse Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0042-CN-F(E)) | FC, WB, ELISA | Mouse Fab |
| HPAB-0043-CN-F(E) | Mouse Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0043-CN-F(E)) | FC, WB, ELISA | Mouse Fab |
| HPAB-0044-CN-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0044-CN-F(E)) | FC, WB, ELISA | Human Fab |
| HPAB-0646-CN-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0646-CN-F(E)) | WB, ELISA, FC | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0646-CN | Human Anti-MSLN Recombinant Antibody (HPAB-0646-CN) | WB, ELISA, FC | Human IgG1 |
| HPAB-2427LY | Human Anti-MSLN Recombinant Antibody (HPAB-2427LY) | ELISA, FC, SPR | Human IgG |
| HPAB-2428LY | Human Anti-MSLN Recombinant Antibody (HPAB-2428LY) | ELISA, FC, SPR | Human IgG |
| HPAB-1338-FY | Human Anti-MSLN Recombinant Antibody (HPAB-1338-FY) | ELISA, FC | Human IgG |
| HPAB-1339-FY | Human Anti-MSLN Recombinant Antibody (HPAB-1339-FY) | ELISA, FC | Human IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-201 | Afuco™ Anti-MSLN ADCC Recombinant Antibody (Amatuximab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
MHC Tetramer for Cancer
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0630-YJ-S(P) | Mouse Anti-MSLN Recombinant Antibody (clone K1); scFv Fragment | ELISA, IF | Mouse scFv |
| HPAB-S0098-YC-S(P) | Mouse Anti-MSLN Recombinant Antibody (clone SS); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0257WJ-S(P) | Human Anti-MSLN Recombinant Antibody (clone H1a050); scFv Fragment | ELISA, WB, FC | Human scFv |
| FAMAB-0258WJ-S(P) | Human Anti-MSLN Recombinant Antibody (clone H1a064); scFv Fragment | ELISA, WB, FC | Human scFv |
| FAMAB-0259WJ-S(P) | Human Anti-MSLN Recombinant Antibody (clone H1a021); scFv Fragment | ELISA, WB, FC | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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