Rabbit Anti-MSN (pT558) Recombinant Antibody (clone R02-3A8)
CAT#: VS3-WK2034
This product is a rabbit recombinant antibody that is specific to phosphorylated MSN. The target of this antibody is phospho-MSN (Thr558). The clone R02-3A8 can be used in WB analysis at a dilution of 1/500-1/1000 and IHC staining at a dilution of 1/50-1/100. The molecular weight of MSN (pThr558) observed in WB experiments using this antibody is 68 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10898/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47336/if_selected.jpg
Normal Tissue
Figure 3 Lung
Alveolar cells type I Staining: Low Intensity: Moderate Quantity: <25% Alveolar cells type I I Staining: Medium Intensity: Moderate Quantity: 75%-25% Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Macrophages Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47338/115899_A_1_4.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47338/115899_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47338/115899_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47338/115899_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147065-MSN
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WB
Figure 1 Rabbit Anti-MSN (pThr558) Recombinant Antibody (clone R02-3A8) in WB.
Western blot analysis of Moesin (phospho-Thr558) in Hela, A549, HL-60, C6 lysates using anti-MSN (pThr558) antibody, Cat# VS3-WK2034.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Thr558 of human Moesin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr558 of human and rat MSN
- Species Reactivity
- Human, Rat
- Clone
- R02-3A8
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "MSN"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2005z | Mouse Anti-MSN Recombinant Antibody (clone 9C10) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MRO-1015-CN | Recombinant Rabbit Anti-MSN Monoclonal Antibody (CBACN-379) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY965 | Recombinant Rabbit Anti-MSN Antibody (clone R08-3G5) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS-0923-FY117 | Recombinant Mouse Anti-MSN Antibody (VS-0923-FY117) | ELISA, WB | Mouse IgG1 |
| VS3-FY2809 | Rabbit Anti-MSN Recombinant Antibody (VS3-FY2809) | WB, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2304 | Hi-Affi™ Recombinant Rabbit Anti-MSN Monoclonal Antibody (DS2304AB) | IHC-FoFr, WB, IP, ICC, FC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC249 | AbPlus™ Anti-MSN Magnetic Beads (VS-0724-YC249) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY326 | Mouse Anti-NHP MSN Recombinant Antibody (clone 2C12) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1380 | Anti-MSN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4538 | Anti-Mouse MSN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4539 | Anti-Rat MSN Immunohistochemistry Kit | IHC | |
| VS-0525-XY4537 | Anti-Human MSN Immunohistochemistry Kit | IHC |
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