Rabbit Anti-MST1 Recombinant Antibody (clone R04-1D6)
CAT#: VS3-FY1947
The R04-1D6 recombinant monoclonal antibody is capable of recognizing human MST1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The specificity of the MST1 antibody was confirmed by western blot analysis of MST1 in Hela, C6, rat brain lysates and immunohistochemistry analysis of paraffin-embedded human colon cancer antigen retrieval.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human liver
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes and plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24036/53571_A_9_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24036/53571_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24036/53571_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24036/53571_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24036/53571_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24036/53571_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (Hep G2, NB-4, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173531-MST1
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WB
Figure 1 Anti-MST1 Antibody (VS3-FY1947) in WB.
The MST1 expression in Hela, C6, rat Brain lysates were detected using the anti-MST1 by western blot assay.
IHC
Figure 2 Anti-MST1 Antibody (VS3-FY1947) in IHC.
Immunohistochemistry staining of paraffin-embedded human colon cancer using the anti-MST1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human MST1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MST1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-1D6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 80 kDa, while the observed molecular weight stands at 96 kDa.
- Related Disease
- Human colon cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Macrophage Stimulating 1; Macrophage-Stimulating Protein; D3F15S2; DNF15S2; HGFL; MSP; Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like)
- Gene ID
- 4485
- UniProt ID
- P26927
- Sequence Similarities
- Belongs to the peptidase S1 family. Plasminogen subfamily.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Cleaved after Arg-483, probably by HPN/Hepsin, to yield the active form consisting of two disulfide-linked chains. (P26927-HGFL_HUMAN)
Glycosylation at Asn72, Asn155, Asn238, Asn296, and Asn615 (NX_P26927 [NX_P26927-1])
Modification sites at PhosphoSitePlus (P26927)
Glycosylation from GlyConnect HGFL_HUMAN (1310)
Glycosylation from GlyGen (P26927) 6 sites, 6 N-linked glycans (5 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011532040.1; XP_016861949.1; XP_047304114.1; XP_047304115.1; XP_047304116.1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MST1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2009z | Mouse Anti-MST1 Recombinant Antibody (clone 13H5) | WB, IHC | Mouse IgG1 |
| HPAB-AP149-YC | Human Anti-MST1 Recombinant Antibody (clone chC15.2) | ELISA, Block, Neut | Human IgG |
| VS3-XY3436 | Rabbit Anti-MST1 Recombinant Antibody (clone R01-4B7) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1754CQ | Recombinant Rat Anti-Mst1 Antibody (CBL648) | IHC, Neut | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1755CQ | Recombinant Mouse Anti-MST1 Antibody (CBL305) | BL, WB | IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2307 | Hi-Affi™ Recombinant Rabbit Anti-MST1 Monoclonal Antibody (DS2307AB) | WB, IHC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP149-YC-S(P) | Human Anti-MST1 Recombinant Antibody (clone chC15.2); scFv Fragment | ELISA, Block, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP149-YC-F(E) | Human Anti-MST1 Recombinant Antibody (clone chC15.2); Fab Fragment | ELISA, Block, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4546 | Anti-MST1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4547 | Anti-Mouse MST1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4548 | Anti-Rat MST1 Immunohistochemistry Kit | IHC |
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