Human Anti-MST1R Recombinant Antibody (clone IMC-41A10) (CAT#: HPAB-0266CQ)

Figure 1 Solid-phase binding and blocking characteristics of IMC-41A10.

ELISA to determine whether IMC-41A10 can bind to immobilized recombinant human c-MET protein. An antihuman HGFR (c-MET) antibody is used as a positive control.

Ludwig, D. L., Rabenau, K. E., Hicklin, D. J., Balderes, P., Zhu, Z., & Prewett, M., et al. (2006). Therapeutic implications of a human neutralizing antibody to the macrophage-stimulating protein receptor tyrosine kinase (ron), a c-met family member. Cancer Research, 66(18), 9162.

Figure 2 Inhibition of MSP-dependent receptor and MAPK phosphorylation.

A, effect of IMC-41A10 on MSP-induced phosphorylation of RON. RON-NIH3T3 cells were serum starved (lane 1) and stimulated with MSP (lanes 2, 4, and 5). Lanes 3 and 4, effect of adding IMC-41A10 in the absence and presence of MSP, respectively. Lane 5, effect of adding IMC-42E12, an antibody that binds to RON but does not block MSP binding, in the presence of MSP. B, effect of IMC-41A10 on MSP-induced phosphorylation of MAPK in cell lines representing six types of cancer. These lines were serum starved (lane 1) and stimulated with MSP (lane 2). Lanes 3 and 4, effect of adding IMC-41A10 in comparison with IMC-42E12, respectively.

Ludwig, D. L., Rabenau, K. E., Hicklin, D. J., Balderes, P., Zhu, Z., & Prewett, M., et al. (2006). Therapeutic implications of a human neutralizing antibody to the macrophage-stimulating protein receptor tyrosine kinase (ron), a c-met family member. Cancer Research, 66(18), 9162.

Figure 3 Ability of IMC-41A10 to inhibit cell migration.

B, H&E staining of H596 lung cancer cells that have adhered to and migrated through to the underside of the collagen in the presence of IMC-41A10 and IMC-42E12, an antibody that binds to RON but does not block MSP binding. C, in vitro wound healing assay. A scratch was made in a monolayer of HCA7 colon cancer cells. IMC-41A10 was added to determine whether it could inhibit the ability of MSP to induce the migration of cells to fill the wound.

Ludwig, D. L., Rabenau, K. E., Hicklin, D. J., Balderes, P., Zhu, Z., & Prewett, M., et al. (2006). Therapeutic implications of a human neutralizing antibody to the macrophage-stimulating protein receptor tyrosine kinase (ron), a c-met family member. Cancer Research, 66(18), 9162.