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MTHFR
Rabbit Anti-MTHFR Recombinant Antibody (clone R02-6H3)

Rabbit Anti-MTHFR Recombinant Antibody (clone R02-6H3) (CAT#: VS3-FY1777)

Name: Rabbit Anti-MTHFR Recombinant Antibody (clone R02-6H3)
SKU: VS3-FY1777
Rating: 5 (1 reviews)

Clone R02-6H3 is a rabbit recombinant monoclonal antibody that is capable of recognizing human MTHFR. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This MTHFR antibody has been teted positive by western blot analysis of MTHFR in K562 lysates.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Anti-MTHFR Antibody (VS3-FY1777) in WB.

Figure 1 Anti-MTHFR Antibody (VS3-FY1777) in WB.

The MTHFR expression in K562 lysates were detected using the anti-MTHFR by western blot assay.

Subcellular Location

Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63389/1233_G5_1_selected.jpg

Normal Tissue

Figure 2 Epididymis
(High expression in epididymis)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76180/167458_A_3_6_val_selected.jpg

Figure 3 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/77255/166620_A_6_6.jpg

Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/77255/166620_A_7_3.jpg

Figure 5 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/77255/166620_A_9_4.jpg

Figure 6 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/77255/166620_A_8_5.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enhanced (K-562)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177000-MTHFR

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Recombinant human MTHFR protein

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human and Mouse MTHFR

Species Reactivity
Human, Mouse

Clone
R02-6H3

Applications
Western Blot, Immunofluorescence, Immunoprecipitation

MW
The calculated weight of the target protein is approximately 75 kDa, while the observed molecular weight stands at 75 kDa.

Related Disease
Cancer

Product Property

Purification
Affinity Purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
Methylenetetrahydrofolate Reductase; EC 1.5.1.20; 5,10-Methylenetetrahydrofolate Reductase (NADPH); Methylenetetrahydrofolate Reductase (NAD(P)H)

Gene ID
4524

UniProt ID
P42898

Sequence Similarities
Belongs to the methylenetetrahydrofolate reductase family.

Cellular Localization
Cytosol

Post Translation Modifications
Phosphorylation of an N-terminal serine-rich phosphorylation region increases sensitivity to S-adenosylmethionine and inhibition.

Protein Refseq
XP_005263519.1; XP_005263520.1; XP_011539798.1; XP_016856817.1; XP_047277130.1

Function
Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine.
Represents a key regulatory connection between the folate and methionine cycles (Probable).

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "MTHFR"

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IgG Antibody Products

CAT	Product Name	Application	Type
MOB-1360z	Mouse Anti-MTHFR Recombinant Antibody (clone 27G10)	ELISA, FC, ICC, IF, IHC, WB	Mouse IgG1
MOB-0936MZ	Recombinant Mouse Anti-Human MTHFR Antibody	ELISA	Mouse antibody
ZG-0293C	Mouse Anti-MTHFR Recombinant Antibody (ZG-0293C)	WB, IHC, ELISA	Mouse IgG
VS3-WK79	Mouse Anti-MTHFR Recombinant Antibody (clone F3-D5)	WB, ELISA	Mouse IgG1
VS3-XY1121	Mouse Anti-MTHFR Recombinant Antibody (clone 5D3)	ELISA, WB, IHC	Mouse IgG1

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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