Rabbit Anti-MTHFR Recombinant Antibody (clone R02-6H3)
CAT#: VS3-FY1777
Clone R02-6H3 is a rabbit recombinant monoclonal antibody that is capable of recognizing human MTHFR. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This MTHFR antibody has been teted positive by western blot analysis of MTHFR in K562 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63389/1233_G5_1_selected.jpg
Normal Tissue
Figure 2 Epididymis
High expression in epididymis
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76180/167458_A_3_6_val_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77255/166620_A_6_6.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77255/166620_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77255/166620_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77255/166620_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (K-562)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177000-MTHFR
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WB
Figure 1 Anti-MTHFR Antibody (VS3-FY1777) in WB.
The MTHFR expression in K562 lysates were detected using the anti-MTHFR by western blot assay.
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Specifications
- Immunogen
- Recombinant human MTHFR protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MTHFR
- Species Reactivity
- Human, Mouse
- Clone
- R02-6H3
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 75 kDa, while the observed molecular weight stands at 75 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Methylenetetrahydrofolate Reductase; EC 1.5.1.20; 5,10-Methylenetetrahydrofolate Reductase (NADPH); Methylenetetrahydrofolate Reductase (NAD(P)H)
- Gene ID
- 4524
- UniProt ID
- P42898
- Sequence Similarities
- Belongs to the methylenetetrahydrofolate reductase family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Phosphorylation of an N-terminal serine-rich phosphorylation region increases sensitivity to S-adenosylmethionine and inhibition.
- Protein Refseq
- XP_005263519.1; XP_005263520.1; XP_011539798.1; XP_016856817.1; XP_047277130.1
- Function
- Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine.
Represents a key regulatory connection between the folate and methionine cycles (Probable).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MTHFR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1360z | Mouse Anti-MTHFR Recombinant Antibody (clone 27G10) | ELISA, FC, ICC, IF, IHC, WB | Mouse IgG1 |
| MOB-0936MZ | Recombinant Mouse Anti-Human MTHFR Antibody | ELISA | Mouse antibody |
| ZG-0293C | Mouse Anti-MTHFR Recombinant Antibody (ZG-0293C) | WB, IHC, ELISA | Mouse IgG |
| VS3-WK79 | Mouse Anti-MTHFR Recombinant Antibody (clone F3-D5) | WB, ELISA | Mouse IgG1 |
| VS3-XY1121 | Mouse Anti-MTHFR Recombinant Antibody (clone 5D3) | ELISA, WB, IHC | Mouse IgG1 |
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