Rabbit Anti-MTHFR Recombinant Antibody (clone R02-6H3) (CAT#: VS3-FY1777)
Clone R02-6H3 is a rabbit recombinant monoclonal antibody that is capable of recognizing human MTHFR. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This MTHFR antibody has been teted positive by western blot analysis of MTHFR in K562 lysates.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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WB
Figure 1 Anti-MTHFR Antibody (VS3-FY1777) in WB.
The MTHFR expression in K562 lysates were detected using the anti-MTHFR by western blot assay.
Specifications
- Immunogen
- Recombinant human MTHFR protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MTHFR
- Species Reactivity
- Human, Mouse
- Clone
- R02-6H3
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 75 kDa, while the observed molecular weight stands at 75 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Methylenetetrahydrofolate Reductase; EC 1.5.1.20; 5,10-Methylenetetrahydrofolate Reductase (NADPH); Methylenetetrahydrofolate Reductase (NAD(P)H)
- Gene ID
- 4524
- UniProt ID
- P42898
- Sequence Similarities
- Belongs to the methylenetetrahydrofolate reductase family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Phosphorylation of an N-terminal serine-rich phosphorylation region increases sensitivity to S-adenosylmethionine and inhibition.
- Protein Refseq
- XP_005263519.1; XP_005263520.1; XP_011539798.1; XP_016856817.1; XP_047277130.1
- Function
- Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine.
Represents a key regulatory connection between the folate and methionine cycles (Probable).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1360z | Mouse Anti-MTHFR Recombinant Antibody (clone 27G10) | ELISA, FC, ICC, IF, IHC, WB | Mouse IgG1 |
| ZG-0293C | Mouse Anti-MTHFR Recombinant Antibody (ZG-0293C) | WB, IHC, ELISA | Mouse IgG |
| VS3-WK79 | Mouse Anti-MTHFR Recombinant Antibody (clone F3-D5) | WB, ELISA | Mouse IgG1 |
| VS3-XY1121 | Mouse Anti-MTHFR Recombinant Antibody (clone 5D3) | ELISA, WB, IHC | Mouse IgG1 |
| VS7-0425-WR433 | Mouse Anti-MTHFR Monoclonal Antibody (VS7-0425-WR433) | WB, IHC-P, IF, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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