This product is a recombinant mouse antibody that can recognize MUC1. AR20.5 bound to the MUC1 peptide with a KD of 880 ± 123 nm. AR20.5 binds a 6-amino acid epitope (DTRPAP) within the repeating segment of 20 amino acids in the MUC1 VNTR domain. The AR20.5 epitope contains a single putative glycosylation site at Thr.
Figure 1 Interferon (IFN)-γ enzyme-linked immunospot (ELISPOT) data from patients treated with BrevaRex mAb-AR20.5.
T-cell responses to MUC1 pre- and post-mAb-AR20.5 treatment were assessed for production of IEN-γ by ELISPOT assay in response to stimulation with 31mer MUCI peptide. PBMC obtained from heparinized blood of patients before and after administration of mAb-AR20.5 were isolated on Ficoll-Hypaque gradients and cryopreserved. Cells from pre- and post therapy samples were thawed, washed and stimulated with the 31mer MUCI peptide for 1 week, using autologous PBMC as antigen presenting cells. Cultures were then restimulated for 24 h with MUC1 peptide-pulsed autologous dendritic cells generated in parallel with the stimulated PBMC cultures. MUC1-specific T cells were quantified in an IFN-γ ELISPOT.
De Bono, J. S., Rha, S., Stephenson, J., Schultes, B. C., Monroe, P., Eckhardt, G. S., ... & Rowinsky, E. K. (2004). Phase I trial of a murine antibody to MUC1 in patients with metastatic cancer: evidence for the activation of humoral and cellular antitumor immunity. Annals of oncology, 15(12), 1825-1833.
Figure 2 Antitumor effect of MAb AR20.5 in CB6F1 mice.
CB6Fl mice were immunized four times with MAb AR20.5 or controls (three times prior to tumor inoculation (Days 0.7. and 14), once post-tumor inoculation (Day 28) with 50 ug/mouse, i.v.). Tumors were induced by iniection of 413BCR cells into the tail vein (Day 21). The mice were observe for distress and weight loss and sacrificed when they became moribund. Survival times are plotted for each group (n= 5) and p values calculated by log-rank test.
Qi, W., Schultes, B. C., Liu, D., Kuzma, M., Decker, W., & Madiyalakan, R. (2001). Characterization of an anti-MUC1 monoclonal antibody with potential as a cancer vaccine. Hybridoma and hybridomics, 20(5-6), 313-324.
Figure 3 CDC and ADCC properties of MAb AR20.5.
ZR75-1 cells were labeled with ⁵¹Cr and incubated with MAb AR20.5 or MAb AR20.5 (IgM) or control IgG, (mouse IgG1, MOPC-21) in a range of 0.625 to 20 ug/mL for 1 h at room temperature. Cells were washed once and then incubated with either 15% fresh human serum in complete RPMI 1640 media for 4 h for CDC (A) or with human PBMC in an effector: target ratio of 50:1 for ADCC (B). Supernatants were harvested and tested for ⁵¹Cr release. Specific lysis was calculated by subtracting the spontaneous release from each sample and dividing the results by the corrected makimum release by ZR75-1 cells in the presence of Triton X-100. Mouse IgG1 was used as negative control.
Qi, W., Schultes, B. C., Liu, D., Kuzma, M., Decker, W., & Madiyalakan, R. (2001). Characterization of an anti-MUC1 monoclonal antibody with potential as a cancer vaccine. Hybridoma and hybridomics, 20(5-6), 313-324.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
NAB-2049-VHH | Recombinant Anti-human MUC1 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
HPAB-AP504-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (5-24) | FC | Camel VHH |
HPAB-AP505-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (aMUC1) | ELISA | Camel VHH |
HPAB-0736-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (ER46) | ELISA | Camelid VHH |
HPAB-0737-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (AR-32) | ELISA | Camelid VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H56 | Anti-Human MUC1 Recombinant Antibody (Pemtumomab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG |
TAB-765 | Mouse Anti-MUC1 Recombinant Antibody (clone Nacolomab); Fab Fragment | FC, IP, ELISA, Neut, FuncS, IF, ICC | Mouse Fab (IgG1, κ) |
TAB-0388CL-S(P) | Mouse Anti-Human MUC1 Recombinant Antibody scFv Fragment | WB, IHC | |
TAB-0388CL-F(E) | Mouse Anti-Human MUC1 Recombinant Antibody Fab Fragment | WB, IHC | |
TAB-411MZ | Mouse Anti-MUC1 Recombinant Antibody (TAB-411MZ) | ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-166 | Humanized Anti-MUC1 Recombinant Antibody (clone Cantuzumab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | Humanized (from mouse) IgG1, κ |
TAB-413MZ | Human Anti-MUC1 Recombinant Antibody (TAB-413MZ) | ELISA | Humanized IgG |
TAB-413MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-413MZ-S(P)) | ELISA | Humanized scFv |
TAB-425MZ-S(P) | Anti-Human MUC1 Recombinant Antibody scFv Fragment (huDMB5F3) | FC | Humanized antibody |
TAB-430MZ-S(P) | Anti-Human MUC1 Recombinant Antibody scFv Fragment (BLC595) | ELISA, FC | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-L036E | anti-MUC1 immunotoxin C242 (Fab)-PE | Cytotoxicity assay, Functional assay | |
AGTO-L064E | anti-MUC1 immunotoxin H23 (scFv)-PE | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0278CL | Human Anti-MUC1 Recombinant Antibody (TAB-0278CL) | ELISA, Internalization, BI, FuncS | Human IgG |
TAB-431MZ-F(E) | Anti-Human MUC1 Recombinant Antibody Fab Fragment (PH1) | ELISA, WB, FC, IHC | Human antibody |
TAB-432MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-432MZ-F(E)) | ELISA, FC | Human Fab |
TAB-433MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-433MZ-F(E)) | ELISA, FC | Human Fab |
TAB-434MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-434MZ-F(E)) | ELISA, FC | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-412MZ | Human Anti-MUC1 Recombinant Antibody (TAB-412MZ) | ELISA | Chimeric (mouse/human) IgG |
TAB-412MZ-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-412MZ-S(P)) | ELISA | Mouse scFv |
TAB-412MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-412MZ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-012LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
Gly-120LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-012LC-1 | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-109LC | Recombinant Anti-Human MUC1 Antibody (Fc glycosylation) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-121LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
Gly-122LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
Gly-123LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
Gly-124LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0364MZ | Recombinant Mouse Anti-Human Mucin 1, Cell Surface Associated, FITC-Conjugated Antibody (clone Cer-EP5) | ELISA | Mouse antibody |
PABC-400 | Mouse Anti-MUC1 Recombinant Antibody (clone SM3) | FC, IHC-Fr, IF, ELISA, IHC-P | Mouse IgG1 |
HPAB-0207-YJ | Human Anti-MUC1 Recombinant Antibody (clone Clivatuzumab (hPAM4)) | ELISA, RIA, IHC, Immunoperoxidase Staining, Inhib, FuncS | Humanized IgG, κ |
HPAB-1159WJ | Mouse Anti-MUC1 Recombinant Antibody (clone 12E) | ELISA | Mouse IgG |
HPAB-1160WJ | Mouse Anti-MUC1 Recombinant Antibody (clone 3D) | ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0379MZ | Chicken Anti-MUC1 Polyclonal IgY | IHC, WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC054 | PE-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
MHC-LC781 | A*0201/Human MUC-1 (LLLLTVLTV) MHC Tetramer | FCM | |
MHC-LC1950 | PE-A2/Human MUC1 (NLTISDVSV) MHC Tetramer | FCM | |
MHC-YF272 | A*02:01/Human MUC1 (LLLLTVLTV) MHC Monomer | MHC Multimer | |
MHC-YF570 | A*02:01/Human Mucin (TLAPATEPA) MHC Pentamer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2320 | Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB) | IHC-P, IHC-Fr | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0838LC | Recombinant Mouse Anti-Human MUC1 Antibody (SM3) | ELISA | IgG1 |
EPAF-0581CQ | Recombinant Mouse Anti-Human MUC1 Antibody (B27.29) | Neut, FC | IgG1 |
EPAF-0583CQ | Recombinant Mouse Anti-Human MUC1 Antibody (BC4E549) | Neut, FC | IgG1 |
EPAF-0584CQ | Recombinant Mouse Anti-Human MUC1 Antibody (DF3) | Neut, FC | IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0091-YC-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0091-YC-F(E)) | ELISA, Neut | Mouse Fab |
HPAB-0094-YC-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone 12D10); Fab Fragment | ELISA, FC | Mouse Fab |
HPAB-0872LY-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0872LY-F(E)) | ELISA, WB, FC | Mouse Fab |
HPAB-0873LY-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0873LY-F(E)) | ELISA, WB, FC | Humanized Fab |
HPAB-0874LY-F(E) | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0874LY-F(E)) | ELISA, WB, FC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBC-399 | Mouse Anti-MUC1 Recombinant Antibody (clone AR20.5); scFv Fragment | ELISA, FC, FuncS | Mouse scFv |
PSBC-400 | Mouse Anti-MUC1 Recombinant Antibody (clone SM3); scFv Fragment | FC, IHC-Fr, IF, ELISA, IHC-P | Mouse scFv |
HPAB-AP746-YC-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-AP746-YC-S(P)) | ELISA | Mouse scFv |
HPAB-AP747-YC-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (HPAB-AP747-YC-S(P)) | ELISA | Mouse scFv |
FAMAB-0211JF-S(P) | Human Anti-MUC1 Recombinant Antibody (clone G8); scFv Fragment | ELISA, FuncS | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-H56 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Pemtumomab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-H65 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Sontuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-026ML | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Gatipotuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-166 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Cantuzumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
AFC-TAB-H77 | Afuco™ Human Anti-MUC1 Recombinant Antibody, ADCC Enhanced (AFC-TAB-H77) | ELISA, FC, IP, FuncS, IF | IgG1, κ |
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