Mouse Anti-MUC1 Recombinant Antibody (clone BC2)

Name: Mouse Anti-MUC1 Recombinant Antibody (clone BC2)
SKU: VS-0224-XY95
Rating: 5 (1 reviews)

CAT#: VS-0224-XY95

The mouse recombinant antibody specifically recognizes MUC1 (Mucin 1, cell surface associated) from human. It has been tested by Western blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry-paraffin.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line RPTEC TERT1

Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/1789_B9_13_cr596f381e20667_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1986/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/100_E7_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8855/101_E7_1_blue_red_green.jpg

Normal Tissue Figure 5 Stomach

Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_2_1.jpg

Normal Tissue Figure 6 Colon

Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_9_3.jpg

Normal Tissue Figure 7 Kidney

Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_8_5.jpg

Normal Tissue Figure 8 Testis

Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36/155175_A_6_6.jpg

RNA Expression Figure 9 RNA cell line category: Group enriched (CAPAN-2, OE19, RPTEC TERT1, T-47d)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185499-MUC1

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Specifications

Immunogen
Human milk fat globulin membrane preparation

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human MUC1

Species Reactivity
Human

Clone
BC2

Applications
Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry-paraffin

Related Disease
Tubulointerstitial Kidney Disease; Autosomal Dominant; Syringoma

Product Property

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested in Western blot (1/2000), Immunofluorescence (1/10-1/200), and Immunohistochemistry-paraffin (1/10-1/200).

Target

Alternative Names
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADTKD2; Ca15-3; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Gene ID
4582

UniProt ID
P15941

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0224-XY95.pdf

MSDS

MSDS - VS-0224-XY95.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

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Recommended Dilution Buffer

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Secondary Antibody

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