Mouse Anti-MUC2 Recombinant Antibody (clone 7B8G10) (CAT#: VS7-0425-WR439)

• Immunogen
• Recombinant human MUC2 (AA: 4373-4557) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human MUC2

• Species Reactivity
• Human

• Clone
• 7B8G10

• Molecule Class
• Mucin

• Applications
• IHC, FC

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry-Paraffin: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• MUC2; Mucin-Like Protein; Intestinal Mucin-2; MLP; MUC-2; Mucin 2, Intestinal/Tracheal

• Gene ID
• 4583

• UniProt ID
• Q02817

• Long Name
• Mucin 2, oligomeric mucus/gel-forming

• Cellular Localization
• Secreted

• Post Translation Modifications
• Autocatalytic cleavage; Disulfide bond; Glycoprotein; Phosphoprotein

• Protein Refseq
• NP_002448.5

• Function
• Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (PubMed:17058067, PubMed:19432394, PubMed:33031746).; Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine (PubMed:19432394, PubMed:33031746).; MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange (PubMed:33031746, PubMed:36206754).; Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells (PubMed:36206754).; Binds both Cu2+ and its reduced form, Cu1+, at two juxtaposed binding sites: Cu2+, once reduced to Cu1+ by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site (PubMed:36206754).; MUC2-bound Cu1+ is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells (PubMed:36206754).; Mucin gels store antimicrobial molecules that participate in innate immunity (PubMed:33031746).; Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body (PubMed:33031746).; Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon: a 1 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon (By similarity).; 'Thick' mucus transits along the descending colon and is lubricated by a 2 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus (By similarity).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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