Mouse Anti-MUC2 Recombinant Antibody (clone 7B8G10)
CAT#: VS7-0425-WR439
This product is a mouse IgG1 antibody that binds to human MUC2. The antibody 7B8G10 recognizes specific amino acid domain of human MUC2 (AA: 4373-4557). It can be used for IHC and FC assays.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16275/35623_B_8_5.jpg
Normal Tissue
Figure 2 Small intestine
Endocrine cells Staining: High Intensity: Strong Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16275/35623_A_6_2.jpg
Normal Tissue
Figure 3 High expression in colon.
Endocrine cells Staining: High Intensity: Strong Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16275/35623_A_9_3.jpg
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Specifications
- Immunogen
- Recombinant human MUC2 (AA: 4373-4557) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MUC2
- Species Reactivity
- Human
- Clone
- 7B8G10
- Molecule Class
- Mucin
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MUC2; Mucin-Like Protein; Intestinal Mucin-2; MLP; MUC-2; Mucin 2, Intestinal/Tracheal
- Gene ID
- 4583
- UniProt ID
- Q02817
- Long Name
- Mucin 2, oligomeric mucus/gel-forming
- Cellular Localization
- Secreted
- Post Translation Modifications
- Autocatalytic cleavage; Disulfide bond; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_002448.5
- Function
- Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (PubMed:17058067, PubMed:19432394, PubMed:33031746).; Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine (PubMed:19432394, PubMed:33031746).; MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange (PubMed:33031746, PubMed:36206754).; Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells (PubMed:36206754).; Binds both Cu2+ and its reduced form, Cu1+, at two juxtaposed binding sites: Cu2+, once reduced to Cu1+ by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site (PubMed:36206754).; MUC2-bound Cu1+ is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells (PubMed:36206754).; Mucin gels store antimicrobial molecules that participate in innate immunity (PubMed:33031746).; Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body (PubMed:33031746).; Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon: a 1 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon (By similarity).; 'Thick' mucus transits along the descending colon and is lubricated by a 2 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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