Rabbit Anti-MUT Recombinant Antibody (clone R05-3G4)
CAT#: VS3-FY1768
This R05-3G4 recombinant monoclonal antibody demonstrated a greater affinity to human MUT. It cross-reacts with rat. The antibody is recommended for detection of MUT protein by WB, IHC-P. The antibody has the positive detection of methylmalonyl coenzyme A mutase by western blot analysis of methylmalonyl coenzyme A mutase in Hela, rat brain lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35971/if_selected.jpg
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WB
Figure 1 Anti-MUT Antibody (VS3-FY1768) in WB.
The Methylmalonyl Coenzyme A mutase expression in Hela, rat Brain lysates were detected using the anti-Methylmalonyl Coenzyme A mutase by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human methylmalonyl-CoA mutase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat MUT
- Species Reactivity
- Human, Rat
- Clone
- R05-3G4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 83 kDa.
- Related Disease
- Methylmalonic Aciduria Due To Methylmalonyl-Coa Mutase Deficiency and Methylmalonic Acidemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Methylmalonyl-CoA Mutase; Methylmalonyl Coenzyme A Mutase; Methylmalonyl-CoA Isomerase; EC 5.4.99.2; MCM; Methylmalonyl-CoA Mutase, Mitochondrial; Truncated Methylmalonyl CoA Mutase; Mutant Methylmalonyl CoA Mutase
- Gene ID
- 4594
- UniProt ID
- P22033
- Sequence Similarities
- Belongs to the methylmalonyl-CoA mutase family.
- Cellular Localization
- Cytoplasm, Mitochondrion
- Post Translation Modifications
- Acetylation
Phosphoprotein
- Protein Refseq
- XP_005249200.1; XP_054211495.1; NP_000246.2
- Function
- Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MUT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2326 | Hi-Affi™ Recombinant Rabbit Anti-MUT Monoclonal Antibody (DS2326AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC458 | AbPlus™ Anti-MUT Magnetic Beads (VS-0724-YC458) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1398 | Anti-MUT Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC751 | Rabbit Anti-MUT Recombinant Antibody (VS13-YC751) | WB, IHC-P | Rabbit IgG |
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