Human Anti-mutant p53 Recombinant Antibody (clone F2 scFv (TARl)) (CAT#: HPAB-0273CQ)
This product is a recombinant human antibody that recognizes mutant p53. F2 was capable of binding the common epitope of the mutant p53. TARl is capable of inducing cell death by apoptosis in cancerous cell lines expressing endogenous mutant p53 proteins.
Specific Inquiry
FuncS
Figure 1 depicts the binding of scFv F2 to whole p53 molecules as determined using an ELISA.
FC
Figure 2 depicts the effect of the scFv F2 on apoptosis in p53 null human lung carcinoma cells.
Cells were incubated for 24 hours with scFv-F2 at the following concentrations: 0 nM (untreated cells, Figures 4a and f), 250 nM (Figures 4b and g), 500 nM (Figures 4c and h), 1 μM (Figures 4d and i) and 2 μM (Figures 4e and j). Following 24 hours, the cells were fixed with ethanol, stained with Propidium Iodide and the cell cycle profile was determined.
Apop
Figure 3 depicts the effect of the scFv F2 on apoptosis in p53 null human lung carcinoma cells (H 1299 p53-/-).
Cells were incubated for 24 hours with scFv-F2 at the following concentrations: 0 nM (untreated cells, Figures 4a and f), 250 nM (Figures 4b and g), 500 nM (Figures 4c and h), 1 μM (Figures 4d and i) and 2 μM (Figures 4e and j). Following 24 hours, the cells were fixed with ethanol, stained with Propidium Iodide and the cell cycle profile was determined.
Inhib
Figure 4 depict inhibition of colony formation in lung carcinoma cells expressing the scFv F2 antibody.
FuncS
Figure 5 depicts the anti tumor effect of scFv F2 on human tumor cell xenografts in mice.
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Species Reactivity
- Human, Mouse, Rat, Zebrafish
- Clone
- F2 scFv (TARl)
- Applications
- ELISA, FC, Apop, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Apop, Functional Assay.
Target
- Alternative Names
- mutant p53
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0274CQ | Human Anti-mutant p53 Recombinant Antibody (clone E6) | ELISA, FC, WB | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0273CQ-F(E) | Human Anti-mutant p53 Recombinant Antibody (clone F2 scFv (TARl)); Fab Fragment | ELISA, FC, Apop | Human Fab |
| HPAB-0274CQ-F(E) | Human Anti-mutant p53 Recombinant Antibody (clone E6); Fab Fragment | ELISA, FC | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0273CQ-S(P) | Human Anti-mutant p53 Recombinant Antibody (clone F2 scFv (TARl)); scFv Fragment | ELISA, FC, Apop | Human scFv |
| HPAB-0274CQ-S(P) | Human Anti-mutant p53 Recombinant Antibody (clone E6); scFv Fragment | ELISA, FC | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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