Anti-MYC (clone W24V174) Recombinant Antibody Coupled Liposome (VS-1024-FY175)
CAT#: VS-1024-FY175
The anti-c-Myc antibody conjugated liposome demonstrates good targeting capabilities in the treatment of melanoma, effectively targeting and inhibiting the proliferation of tumor cells. c-Myc is an important transcription factor closely associated with the occurrence and development of tumors. The use of this liposome may enhance therapeutic effects while reducing toxicity to normal cells. Further research will help optimize its clinical application.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66556/1279_E11_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/84/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human caudate
Immunohistochemical staining of human caudate shows distinct nucleolar positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/883_H10_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/879_G9_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_7_5.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_7_5.jpg
Normal Tissue
Figure 11 Cervix
Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_8_3.jpg
Normal Tissue
Figure 12 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Cell line enhanced (RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136997-MYC
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More Infomation
Specifications
- Potential Clinical Applications
- Melanoma
Product Composition
- Clone
- W24V174
- Antibody Type
- IgG
- Antibody Host
- Mouse
- Antibody Reactivity
- Human
- Antibody Description
- This product is a recombinant mouse monoclonal antibody that was specifically designed to bind to the c-Myc protein. c-Myc is a crucial transcription factor that plays a significant role in cell growth, proliferation, and differentiation. Due to its involvement in various cellular processes, dysregulation of c-Myc is often associated with several types of cancers.
Product Property
- Storage
- See in the COA
- Storage Shelf Time
- See in the COA
Target Information
- Target
- MYC
- Introduction
- This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
- Alternative Names
- MYC Proto-Oncogene, BHLH Transcription Factor; V-Myc Avian Myelocytomatosis Viral Oncogene Homolog; Class E Basic Helix-Loop-Helix Protein 39; Transcription Factor P64; Proto-Oncogene C-Myc; BHLHe39; Myc-Related Translation/Localization Regulatory Factor
- Full Name
- MYC Proto-Oncogene, BHLH Transcription Factor
- Gene ID
- 4609
- UniProt ID
- P01106
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by PRKDC (PubMed:1597196).
Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity).
Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence (PubMed:19966300, PubMed:20713526).
Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58 (PubMed:22307329).
Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:8386367).
Dephosphorylation at Ser-62 by protein phosphatase 2A (PPP2CA) promotes its degradation; interaction with PPP2CA is enhanced by AMBRA1 (PubMed:25803737, PubMed:25438055).
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:25775507).
In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation (PubMed:17873522, PubMed:17558397).
In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus (PubMed:17558397, PubMed:25775507).
Also polyubiquitinated by the DCX(TRPC4AP) complex (PubMed:20551172, PubMed:29779948).
Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).
- Protein Refseq
- NP_002458.2
- Function
- Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029).
Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029).
Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029).
Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity).
Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).
Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
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Related Signaling Pathways
BCR Signaling Pathway
Related Diseases
Bladder Cancer
Breast Cancer
Colorectal Cancer
Gastric Cancer
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Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
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| MRO-2313-CN | Recombinant Rabbit Anti-MYC (phosphorylated Ser62) Monoclonal Antibody (CBACN-606) | WB, IF, IHC, IP | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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