Mouse Anti-MYC Recombinant Antibody (clone 5H5G5)
CAT#: ZG-0258U
Mouse anti-MYC antibody (clone 5H5G5) displayed high specificity against the MYC protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-MYC Antibody (ZG-0258U) in WB
Positive WB detected in: Hela whole cell lysate. All lanes: MYC antibody at 1: 500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 49, 51 KDa
Observed band size: 49 KDa
Exposure time: 5min
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66556/1279_E11_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/84/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human caudate
Immunohistochemical staining of human caudate shows distinct nucleolar positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/883_H10_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/879_G9_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_7_5.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_7_5.jpg
Normal Tissue
Figure 11 Cervix
Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_8_3.jpg
Normal Tissue
Figure 12 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Cell line enhanced (RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136997-MYC
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Specifications
- Immunogen
- Recombinant Human MYC protein (1-439AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MYC
- Species Reactivity
- Human
- Clone
- 5H5G5
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Burkitt Lymphoma and High-Grade B-Cell Lymphoma Double-Hit/Triple-Hit.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
- Alternative Names
- MRTL; MYCC; c-Myc; bHLHe39; myc proto-oncogene protein; avian myelocytomatosis viral oncogene homolog; class E basic helix-loop-helix protein 39; myc-related translation/localization regulatory factor; proto-oncogene c-Myc; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
- Gene ID
- 4609
- UniProt ID
- P01106
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Related Signaling Pathways
BCR Signaling Pathway
Related Diseases
Bladder Cancer
Breast Cancer
Colorectal Cancer
Gastric Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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