CytoStream™ Rabbit Anti-MYLK Recombinant Antibody (VS13-YC762)
CAT#: VS13-YC762
The CytoStream™ Rabbit Anti-MYLK Recombinant Antibody is specific for human, mouse and rat MYLK. MYLK encodes myosin light chain kinase, facilitating muscle contractility, with forms across smooth muscle and nonmuscle tissues, producing various isoforms. The anti-MYLK antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31677/1742_C2_13_cr58048fbf3cbb5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48055/1742_D1_4_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20789/47152_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20789/47152_A_7_3.jpg
Normal Tissue
Figure 5 High expression in smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31677/88290_B_5_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-RPE1, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065534-MYLK
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Specifications
- Immunogen
- Recombinant human MYLK, aa 1865-1914.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MYLK
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/10000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Immunohistochemistry-paraffin (IHC-P): 1/100-1/800
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Myosin Light Chain Kinase; Smooth Muscle Myosin Light Chain Kinase; Myosin, Light Polypeptide Kinase; Kinase-Related Protein; EC 2.7.11.18; Telokin; SmMLCK; MLCK1; MYLK1;
- Gene ID
- 4638
- UniProt ID
- Q15746
- Long Name
- Myosin Light Chain Kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
- Cellular Localization
- Cytoplasm; Cell projection; Cleavage furrow; Cytoskeleton
- Protein Refseq
- NP_001308238.1; NP_444253.3; NP_444254.3
- Function
- Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca2+ entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MYLK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3130z | Mouse Anti-MYLK Recombinant Antibody (clone 1H10) | WB, ELISA | Mouse IgG1, κ |
| MRO-1058-CN | Recombinant Rabbit Anti-MYLK Monoclonal Antibody (CBACN-392) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2052-CN | Rabbit Anti-MYLK Polyclonal Antibody (MRO-2052-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-WK996 | Rabbit Anti-MYLK Recombinant Antibody (VS3-WK996) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS7-0425-WR451 | Mouse Anti-MYLK Recombinant Antibody (clone 7F2B11) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0762MZ | Chicken Anti-MYLK Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2349 | Hi-Affi™ Recombinant Rabbit Anti-MYLK Monoclonal Antibody (DS2349AB) | WB, FC, ICC, IF, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1426 | Anti-MYLK Immunohistochemistry Kit | IHC | |
| VS-0525-XY4648 | Anti-Human MYLK Immunohistochemistry Kit | IHC |
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