Mouse Anti-MYOD1 Recombinant Antibody (clone ABT-MYOD1) (CAT#: VS3-CJ664)
This product is a mouse antibody that recognizes human MYOD1.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line LHCN-M2
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55436/1781_C6_34_selected.jpg
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55436/1781_C6_34_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78647/1807_A12_34_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78647/1807_A12_34_selected.jpg
Figure 3 RNA cell line category: Cell line enriched (RH-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129152-MYOD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129152-MYOD1
Specifications
- Immunogen
- Synthesized peptide derived from human MyoD1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MYOD1
- Species Reactivity
- Human
- Clone
- ABT-MYOD1
- Applications
- WB, IF, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunofluorescence (1:500-1:200), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- PUM; MYF3; MYOD; bHLHc1; MYODRIF
- Gene ID
- 4654
- UniProt ID
- P15172
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
- Protein Refseq
- NP_002469.2
- Function
- Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0329MZ | Recombinant Mouse Anti-Human Myogenic Differentiation 1 Antibody (clone 6.9B) | IHC | Mouse antibody |
| MOB-0131F | Mouse Anti-MyoD1 Recombinant Antibody (MOB-0131F) | IHC-P, IF | Mouse IgG |
| MOB-0132F | Mouse Anti-MyoD1 Recombinant Antibody (MOB-0132F) | IHC-P, IF | Mouse IgG |
| VS3-WK545 | Rabbit Anti-MYOD1 Recombinant Antibody (clone JM10-72) | WB, IP, FC | Rabbit IgG |
| VS11-QX115 | Mouse Anti-MYOD1 Recombinant Antibody (clone 6F4-2D7) | IHC, WB, ELISA | Mouse IgG2a, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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