Rabbit Anti-MYOD1 Recombinant Antibody (clone R04-6X8) (CAT#: VS3-XY3442)
This is a recombinant rabbit antibody that recognizes human MYOD1 (Myogenic differentiation 1), which induces fibroblasts to differentiate into myoblasts. It is capable of recognizing human MYOD1. It has been used in a variety of applications, such as Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western Blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line LHCN-M2
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55436/1781_C6_34_selected.jpg
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55436/1781_C6_34_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78647/1807_A12_34_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78647/1807_A12_34_selected.jpg
Figure 3 RNA cell line category: Cell line enriched (RH-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129152-MYOD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129152-MYOD1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MYOD1
- Species Reactivity
- Human
- Clone
- R04-6X8
- Applications
- WB, IHC-P
- Related Disease
- Congenital Myopathy 17; Myopathy, Centronuclear, 1
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- PUM; MYF3; MYOD; CMYP17; bHLHc1; MYODRIF
- Gene ID
- 4654
- UniProt ID
- P15172
- Long Name
- Myogenic differentiation 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
- Protein Refseq
- NP_002469.2
- Function
- Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "MYOD1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2296z | Mouse Anti-MYOD1 Recombinant Antibody (clone 24G3) | FC, IF, IHC | Mouse IgG1, κ |
| MOB-0329MZ | Recombinant Mouse Anti-Human Myogenic Differentiation 1 Antibody (clone 6.9B) | IHC | Mouse antibody |
| ZG-0300C | Mouse Anti-MYOD1 Recombinant Antibody (ZG-0300C) | WB, ELISA | Mouse IgG |
| VS3-FY996 | Recombinant Mouse Anti-MYOD1 Antibody (clone 3B4C3) | ELISA, ICC, FC | Mouse IgG1 |
| VS3-FY997 | Recombinant Mouse Anti-MYOD1 Antibody (clone 3A10-1C10-10D5) | IHC-P | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2352 | Hi-Affi™ Recombinant Rabbit Anti-MYOD1 Monoclonal Antibody (DS2352AB) | WB, IP, FC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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