Rabbit Anti-MYOD1 Recombinant Antibody (clone R04-6X8)
CAT#: VS3-XY3442
This is a recombinant rabbit antibody that recognizes human MYOD1 (Myogenic differentiation 1), which induces fibroblasts to differentiate into myoblasts. It is capable of recognizing human MYOD1. It has been used in a variety of applications, such as Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western Blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line LHCN-M2
Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55436/1781_C6_34_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78647/1807_A12_34_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129152-MYOD1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MYOD1
- Species Reactivity
- Human
- Clone
- R04-6X8
- Applications
- WB, IHC-P
- Related Disease
- Congenital Myopathy 17; Myopathy, Centronuclear, 1
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- PUM; MYF3; MYOD; CMYP17; bHLHc1; MYODRIF
- Gene ID
- 4654
- UniProt ID
- P15172
- Long Name
- Myogenic differentiation 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
- Protein Refseq
- NP_002469.2
- Function
- Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Recommended Dilution Buffer
- CAT
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Secondary Antibody
- CAT
- Product Name
See other products for "MYOD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2296z | Mouse Anti-MYOD1 Recombinant Antibody (clone 24G3) | FC, IF, IHC | Mouse IgG1, κ |
| ZG-0300C | Mouse Anti-MYOD1 Recombinant Antibody (ZG-0300C) | WB, ELISA | Mouse IgG |
| ZG-0730F | Mouse Anti-MYOD1 Recombinant Antibody (clone 5.2F) | ELISA, WB, IP, IF, IHC | Mouse IgG2a |
| ZG-0731F | Mouse Anti-MYOD1 Recombinant Antibody (clone 5.8A) | IHC, ELISA, IP, IF | Mouse IgG1 |
| VS3-CJ664 | Mouse Anti-MYOD1 Recombinant Antibody (clone ABT-MYOD1) | WB, IF, IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2352 | Hi-Affi™ Recombinant Rabbit Anti-MYOD1 Monoclonal Antibody (DS2352AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX798 | Mouse Anti-MYOD1 Recombinant Antibody (clone 598CT21.2.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY196 | AbPlus™ Anti-MYOD1 Magnetic Beads (5.2F) | IP, Protein Purification | |
| VS-0724-YC669 | AbPlus™ Anti-MYOD1 Magnetic Beads (VS-0724-YC669) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4657 | Anti-MYOD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4658 | Anti-Mouse MYOD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4659 | Anti-Porcine MYOD1 Immunohistochemistry Kit | IHC |
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