Rabbit Anti-Myosin Recombinant Antibody (clone 2D6-B1) (CAT#: FAMAB-1397CQ)

This product is a recombinant rabbit antibody that recognizes Myosin. The antibody was purified by affinity chromatography. It can be used in WB, ELISA, IHC.


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ELISA

Figure 1 Competitive ELISA.

Figure 1 Competitive ELISA.

To compare the binding affinity of monoclonal antimyosin antibodies to BALB/c and DBA/2 myosin, titrations of antibody were assayed by ELISA.

Liao, L., Sindhwani, R., Rojkind, M., Factor, S., Leinwand, L., & Diamond, B. (1995). Antibody-mediated autoimmune myocarditis depends on genetically determined target organ sensitivity. The Journal of experimental medicine, 181(3), 1123-1131.

IHC

Figure 2 Heart section from a DBA/2 mouse injected with monoclonal anti-myosin antibody 2D6-B1.

Figure 2 Heart section from a DBA/2 mouse injected with monoclonal anti-myosin antibody 2D6-B1.

There is focal left ventricular myocellular necrosis and mononuclear inflammation diagnostic of active myocarditis. Arrows identify sites of inflammation. (Hematoxylin-eosin, original, x200).

Liao, L., Sindhwani, R., Rojkind, M., Factor, S., Leinwand, L., & Diamond, B. (1995). Antibody-mediated autoimmune myocarditis depends on genetically determined target organ sensitivity. The Journal of experimental medicine, 181(3), 1123-1131.


Specifications

  • Immunogen
  • Cardiac myosin purified from BALB/c mouse hearts
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Mouse cardiac myosin. Cross-reacts with keratin and GlcNAc-BSA.
  • Species Reactivity
  • Mouse
  • Clone
  • 2D6-B1
  • Applications
  • ELISA, IHC

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Immunohistochemistry and Enzyme-Linked Immunosorbent Assay.

Target

  • Alternative Names
  • Myosin-3; Myosin-6; Myosin-7; Myosin-10; Myosin-14; Myosin-11; Myosin-8

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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