Mouse Anti-N Recombinant Antibody (clone 9B12F2) (CAT#: ZG-0271U)

Mouse anti-SARS-CoV-2 N antibody (clone 9B12F2) displayed high specificity against the N protein of Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV).


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WB

Figure 1 Mouse Anti-N Antibody (ZG-0271U) in WB

Figure 1 Mouse Anti-N Antibody (ZG-0271U) in WB

Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli at 100ng All lanes N antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 18 KDa
Observed band size: 18 KDa

ELISA

Figure 2 Mouse Anti-N Antibody (ZG-0271U) in ELISA

Figure 2 Mouse Anti-N Antibody (ZG-0271U) in ELISA

The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 15.36 to 23.97 ng/ml.


Specifications

  • Immunogen
  • Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) N
  • Species Reactivity
  • Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • Clone
  • 9B12F2
  • Applications
  • ELISA, WB
  • Conjugate
  • Unconjugated
  • Related Disease
  • Human Novel Coronavirus.

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
    WB:1000-5000, ELISA:1:1000-1:5000

Target

  • Alternative Names
  • Nucleocapsid protein; NC; protein N; N; NTD

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Human Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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