Mouse Anti-N Recombinant Antibody (clone 9B12F2)
CAT#: ZG-0271U
Mouse anti-SARS-CoV-2 N antibody (clone 9B12F2) displayed high specificity against the N protein of Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV).
Tested Data
WB
Figure 1 Mouse Anti-N Antibody (ZG-0271U) in WB
Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli at 100ng All lanes N antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 18 KDa
Observed band size: 18 KDa
ELISA
Figure 2 Mouse Anti-N Antibody (ZG-0271U) in ELISA
The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 15.36 to 23.97 ng/ml.
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Specifications
- Immunogen
- Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) N
- Species Reactivity
- Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
- Clone
- 9B12F2
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Human Novel Coronavirus.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1000-5000, ELISA:1:1000-1:5000
Target
- Alternative Names
- Nucleocapsid protein; NC; protein N; N; NTD
- UniProt ID
- P0DTC9
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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