Mouse Anti-NANOG Monoclonal Antibody (VS7-0425-WR455) (CAT#: VS7-0425-WR455)
This product is a mouse IgG antibody that binds to human NANOG. The antibody recognizes specific amino acid domain of Nanog (aa20-166). It can be used for WB, IF, ICC, and ELISA assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SuSa
(Immunofluorescent staining of human cell line SuSa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72117/1968_G5_3_selected.jpg
(Immunofluorescent staining of human cell line SuSa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72117/1968_G5_3_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows weak nuclear positivity in subset of cells in seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/ihc_selected.jpg
(Immunohistochemical staining of human testis shows weak nuclear positivity in subset of cells in seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_B_9_5.jpg
Figure 4 Colon
(Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_A_9_3.jpg
(Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_A_9_3.jpg
Figure 5 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_A_6_6.jpg
Figure 6 RNA cell line category: Cell line enriched (NTERA-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111704-NANOG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111704-NANOG
Specifications
- Immunogen
- Recombinant Nanog (aa20-166) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NANOG
- Species Reactivity
- Human
- Molecule Class
- Dna Binding Homeobox Transcription Factor
- Applications
- WB, IF, ICC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NANOG; Nanog Homeobox; Homeobox Transcription Factor Nanog; HNanog
- Gene ID
- 79923
- UniProt ID
- Q9H9S0
- Long Name
- Nanog Homeobox
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001284627.1
- Function
- Activator; Developmental protein; DNA-binding; Repressor
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4004z | Mouse Anti-NANOG Recombinant Antibody (clone 4A8) | WB, ICC, IF, ELISA | Mouse IgG1 |
| MRO-1061-CN | Recombinant Rabbit Anti-NANOG Monoclonal Antibody (CBACN-395) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2055-CN | Rabbit Anti-NANOG Polyclonal Antibody (MRO-2055-CN) | WB | Rabbit IgG |
| ZG-0235U | Mouse Anti-NANOG Recombinant Antibody (clone 8A1D11) | ELISA, WB, ICC, IF, FC | Mouse IgG1 |
| VS3-QX800 | Mouse Anti-NANOG Recombinant Antibody (clone 1E6C4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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