Mouse Anti-NANOG Monoclonal Antibody (VS7-0425-WR455)
CAT#: VS7-0425-WR455
This product is a mouse IgG antibody that binds to human NANOG. The antibody recognizes specific amino acid domain of Nanog (aa20-166). It can be used for WB, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SuSa
Immunofluorescent staining of human cell line SuSa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72117/1968_G5_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows weak nuclear positivity in subset of cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (NTERA-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111704-NANOG
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Specifications
- Immunogen
- Recombinant Nanog (aa20-166) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NANOG
- Species Reactivity
- Human
- Molecule Class
- Dna Binding Homeobox Transcription Factor
- Applications
- WB, IF, ICC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NANOG; Nanog Homeobox; Homeobox Transcription Factor Nanog; HNanog
- Gene ID
- 79923
- UniProt ID
- Q9H9S0
- Long Name
- Nanog Homeobox
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001284627.1
- Function
- Activator; Developmental protein; DNA-binding; Repressor
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NANOG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2363 | Hi-Affi™ Recombinant Rabbit Anti-NANOG Monoclonal Antibody (DS2363AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2055-CN | Rabbit Anti-NANOG Polyclonal Antibody (MRO-2055-CN) | WB | Rabbit IgG |
| ZG-0303C | Mouse Anti-NANOG Recombinant Antibody (ZG-0303C) | WB, IF, ELISA | Mouse IgG |
| ZG-0235U | Mouse Anti-NANOG Recombinant Antibody (clone 8A1D11) | ELISA, WB, ICC, IF, FC | Mouse IgG1 |
| VS3-QX800 | Mouse Anti-NANOG Recombinant Antibody (clone 1E6C4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK1160 | Rabbit Anti-NANOG Recombinant Antibody (VS3-WK1160) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC16 | Mouse Anti-NANOG Recombinant Antibody (VS-0924-YC16) - Cancer Stem Cell Marker | IHC, WB, IF |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC765 | CytoStream™ Rabbit Anti-NANOG Recombinant Antibody (VS13-YC765) | WB, ICC, IF, FC, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4683 | Anti-NANOG Immunohistochemistry Kit | IHC | |
| VS-0525-XY4684 | Anti-Mouse NANOG Immunohistochemistry Kit | IHC |
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