Mouse Anti-NANOG Recombinant Antibody (VS-0924-YC16) - Cancer Stem Cell Marker
CAT#: VS-0924-YC16
This product is an antibody panel that includes the anti-NANOG recombinant antibody (VS-0924-YC73) and the anti-NANOG recombinant antibody (VS-0924-YC74). These antibodies target the human NANOG protein, a marker for cancer stem cells (CSCs). The monoclonal antibodies (mAbs) can be used to detect NANOG in CSC tissues or cells through various methods: Immunohistochemistry (1:1000-1:2500), Immunofluorescence (2-10 µg/mL), and Western Blot (1 µg/mL).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SuSa
Immunofluorescent staining of human cell line SuSa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72117/1968_G5_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows weak nuclear positivity in subset of cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19380/152757_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (NTERA-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111704-NANOG
❮
❯
| Sub Cat | Type | Applications | Concentration | |
| VS-0924-YC73 | Mouse IgG2a | IHC, IF | 1.0 mg/mL | |
| VS-0924-YC74 | Mouse IgG1 | IHC, WB, IF | 0.5 mg/mL |
Specifications
- Host Species
- Mouse
- Specificity
- Human NANOG
- Species Reactivity
- Human
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/1000-1/2500
Western Blot (WB): 1 µg/mL
Immunofluorescence (IF): 2-10 µg/mL
Target
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NANOG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4004z | Mouse Anti-NANOG Recombinant Antibody (clone 4A8) | WB, ICC, IF, ELISA | Mouse IgG1 |
| ZG-0303C | Mouse Anti-NANOG Recombinant Antibody (ZG-0303C) | WB, IF, ELISA | Mouse IgG |
| ZG-0235U | Mouse Anti-NANOG Recombinant Antibody (clone 8A1D11) | ELISA, WB, ICC, IF, FC | Mouse IgG1 |
| VS3-WK1160 | Rabbit Anti-NANOG Recombinant Antibody (VS3-WK1160) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS7-0425-WR455 | Mouse Anti-NANOG Monoclonal Antibody (VS7-0425-WR455) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2363 | Hi-Affi™ Recombinant Rabbit Anti-NANOG Monoclonal Antibody (DS2363AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC765 | CytoStream™ Rabbit Anti-NANOG Recombinant Antibody (VS13-YC765) | WB, ICC, IF, FC, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4683 | Anti-NANOG Immunohistochemistry Kit | IHC | |
| VS-0525-XY4684 | Anti-Mouse NANOG Immunohistochemistry Kit | IHC |
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