Rabbit Anti-NANOS1 Recombinant Antibody (clone R49-9G-1)
CAT#: VS3-FY2109
Clone R49-9G-1 is a rabbit IgG recombinant monoclonal antibody that demonstrated a greater affinity to human NANOS1 protein, a calculated weight of 161 kDa. It cross-reacts with mouse, rat. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20), flow cytometry (dilution 1/50).
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NANOS1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R49-9G-1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 161 kDa, while the observed molecular weight stands at 100-150kDa.
- Related Disease
- Spermatogenic Failure 12 and Male Infertility With Azoospermia Or Oligozoospermia Due To Single Gene Mutation
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Flow Cytometry: 1/50
Target
- Alternative Names
- Nanos C2HC-Type Zinc Finger 1; EC_Rep1a; NOS-1; NOS1; Nanos Homolog 1 (Drosophila); Nanos Homolog 1; ZC2HC12A; SPGF12
- Gene ID
- 340719
- UniProt ID
- Q8WY41
- Sequence Similarities
- Belongs to the nanos family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q8WY41)
Modification sites at neXtProt (NX_Q8WY41)
- Protein Refseq
- NP_955631.1
- Function
- May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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