Mouse Anti-NDC80 Recombinant Antibody (clone 4B12H3)
CAT#: VS7-0425-WR459
This product is a mouse IgG1 antibody that binds to human NDC80. The antibody 4B12H3 recognizes specific amino acid domain of human NDC80 (AA: 443-642). It can be used for WB, IHC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66330/1754_H11_32_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000080986-NDC80
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Specifications
- Immunogen
- Recombinant human NDC80 (AA: 443-642) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NDC80
- Species Reactivity
- Human
- Clone
- 4B12H3
- Molecule Class
- Heterotetrameric Protein Complex
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NDC80; Kinetochore Protein Hec1; Highly Expressed In Cancer, Rich In Leucine Heptad Repeats; HEC; Kinetochore-Associated Protein 2; NDC80 Homolog, Kinetochore Complex Component; KNTC2; HEC1; Kinetochore Protein NDC80 Homolog; HsNDC80; NDC80 Kinetochore Co
- Gene ID
- 10403
- UniProt ID
- O14777
- Long Name
- NDC80 kinetochore complex component
- Cellular Localization
- Centromere; Chromosome; Kinetochore; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_006092.1
- Function
- Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327, PubMed:30409912, PubMed:9315664).; Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592, PubMed:30409912).; The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).; Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:23891108, PubMed:25743205).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2387 | Hi-Affi™ Recombinant Rabbit Anti-NDC80 Monoclonal Antibody (DS2387AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY244 | Mouse Anti-NHP NDC80 Recombinant Antibody (clone 9G3.23) | WB, IF, IP | Mouse IgG2a |
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