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    Rabbit Anti-NDUFA12 Recombinant Antibody (clone 2506W22) (CAT#: VS-0625-FY52)

    The Rabbit IgG monoclonal antibody, clone 2506W22, broadly targets NDUFA12 in Human, Mouse, and Rat. Its immunogen is a recombinant Rat NDUFA12 fusion protein, with an epitope mapping to amino acids 321-353. With an approximate antigen size of 45 kDa, this antibody is applicable in Western Blot, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin), offering versatile detection.

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    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/39903/464_A8_2_selected.jpg
    Normal Tissue
    Figure 2 Cerebral cortex
    Figure 2 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/39903/87061_B_7_5.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/39903/87061_A_9_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/39903/87061_A_7_4.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/39903/87061_A_9_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/39903/87061_A_6_6.jpg
    Figure 7 Heart muscle
    Figure 7 Heart muscle
    (Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/39903/87061_B_4_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/39903/87061_A_8_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184752-NDUFA12

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • 2506W22
    • Applications
    • Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry-Paraffin
    • Related Disease
    • Mitochondrial Complex I Deficiency (various forms).

    Product Property

    • Purification
    • Purified with Protein A or G affinity chromatography
    • Purity
    • >90% as determined by SDS-PAGE
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • 59% PBS pH 7.2-7.4, 40% glycerol (glycerin, glycerine), 0.05% BSA
    • Preservative
    • 0.01% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Optimal dilutions for assays should be determined by the user.

    Target

    • Alternative Names
    • NADH:Ubiquinone Oxidoreductase Subunit A12; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 12; NADH-Ubiquinone Oxidoreductase Subunit B17.2; 13 KDa Differentiation-Associated Protein; Complex I B17.2 Subunit; CI-B17.2
    • Long Name
    • NADH:Ubiquinone Oxidoreductase Subunit A12

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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