Rabbit Anti-NDUFAF5 Recombinant Antibody (clone R01-3D7)
CAT#: VS3-FY1789
This R01-3D7 recombinant monoclonal antibody has been genetically engineered to specifically target human NDUFAF5. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The specificity of the NDUFAF5 antibody was confirmed by western blot analysis of NDUFAF5 in 3T3 lysates.
Tested Data
WB
Figure 1 Anti-NDUFAF5 Antibody (VS3-FY1789) in WB.
The NDUFAF5 expression in 3T3 lysates were detected using the anti-NDUFAF5 by western blot assay.
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Specifications
- Immunogen
- Recombinant human NDUFAF5 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse NDUFAF5
- Species Reactivity
- Human, Mouse
- Clone
- R01-3D7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 39 kDa.
- Related Disease
- Mitochondrial Complex I Deficiency, Nuclear Type 16 and Leigh Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 5; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 5; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 5; Putative Methyltransferase NDUFAF5; C20orf7; Probable Methyltransferase C20orf7, Mitochondrial; Arginine-Hydroxylase NDUFAF5, Mitochondrial
- Gene ID
- 79133
- UniProt ID
- Q5TEU4
- Sequence Similarities
- Belongs to the methyltransferase superfamily.
- Cellular Localization
- Membrane, Mitochondrion, Mitochondrion inner membrane
- Post Translation Modifications
- Ubiquitination at Lys109 (NX_Q5TEU4 [NX_Q5TEU4-1])
Modification sites at PhosphoSitePlus (Q5TEU4)
- Protein Refseq
- XP_006723687.1; XP_011527644.1; XP_047296425.1; XP_047296426.1; XP_047296427.1
- Function
- Acts by mediating hydroxylation of 'Arg-111' of NDUFS7.
May also have methyltransferase activity (Probable).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NDUFAF5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4737 | Anti-NDUFAF5 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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