Rabbit Anti-NDUFB9 Recombinant Antibody (clone R05-9F9)
CAT#: VS3-FY1793
Clone R05-9F9 is a rabbit recombinant monoclonal antibody that has the ability to specifically bind to human NDUFB9. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This NDUFB9 antibody is guaranteed for western blot analysis of NDUFB9 in rat brain lysates.
Tested Data
WB
Figure 1 Anti-NDUFB9 Antibody (VS3-FY1793) in WB.
The NDUFB9 expression in rat Brain lysates were detected using the anti-NDUFB9 by western blot assay.
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Specifications
- Immunogen
- Recombinant human NDUFB9 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NDUFB9
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-9F9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 22 kDa.
- Related Disease
- Lethal neonatal disease, Hypertrophic cardiomyopathy, Liver disease, and adult-onset neurodegenerative disorders
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Subunit B9; NADH Dehydrogenase (Ubiquinone) 1 Beta Subcomplex, 9, 22kDa; NADH-Ubiquinone Oxidoreductase B22 Subunit; LYR Motif-Containing Protein 3; Complex I B22 Subunit; CI-B22; UQOR22
- Gene ID
- 4715
- UniProt ID
- Q9Y6M9
- Sequence Similarities
- Belongs to the complex I LYR family.
- Cellular Localization
- Membrane, Mitochondrion, Mitochondrion inner membrane
- Post Translation Modifications
- Ubiquitination at Lys15 (NX_Q9Y6M9 [NX_Q9Y6M9-1])
Modification sites at PhosphoSitePlus (Q9Y6M9)
Glycosylation from GlyGen (Q9Y6M9) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001265574.1; NP_001265575.1; NP_001298097.1; NP_004996.1
- Function
- Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NDUFB9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY337 | Mouse Anti-NHP NDUFB9 Recombinant Antibody (clone OTI8B7) | WB, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4744 | Anti-NDUFB9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4745 | Anti-Mouse NDUFB9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4746 | Anti-Rat NDUFB9 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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