Rabbit Anti-NECTIN2 Recombinant Antibody (clone R06-8F3) (CAT#: VS3-FY2044)
The R06-8F3 recombinant monoclonal antibody has the ability to specifically bind to human NECTIN2. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. The nectin 2 antibody is recommended for detection of target protein by western blot analysis of nectin 2 in Hela, A549, HL-60, U251, U87-MG lysates.
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WB
Figure 1 Anti-NECTIN2 Antibody (VS3-FY2044) in WB.
The Nectin 2 expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-Nectin 2 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human Nectin 2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NECTIN2
- Species Reactivity
- Human
- Clone
- R06-8F3
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 58 kDa, while the observed molecular weight stands at 70-80 kDa.
- Related Disease
- Herpes Simplex and Cleft Lip/Palate-Ectodermal Dysplasia Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Nectin Cell Adhesion Molecule 2; Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B); PVRL2; HVEB; PRR2; Poliovirus Receptor-Related Protein 2; Herpes Virus Entry Mediator B; Herpesvirus Entry Mediator B
- Gene ID
- 5819
- UniProt ID
- Q92692
- Sequence Similarities
- Belongs to the nectin family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Glycosylation at Ser95, Asn137, and Asn324 (NX_Q92692 [NX_Q92692-1])
Modification sites at PhosphoSitePlus (Q92692)
Glycosylation from GlyConnect NECT2_HUMAN (1537)
Glycosylation from GlyGen (Q92692) 7 sites, 2 N-linked glycans (1 site), 2 O-linked glycans (5 sites)
- Protein Refseq
- XP_047295125.1; XP_054177604.1; NP_001036189.1; NP_002847.1
- Function
- Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive.
Probable cell adhesion protein.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAPAB-0338-CN | Recombinant Mouse Anti-Nectin2 Antibody | WB | Mouse IgG |
| MRO-1070-CN | Recombinant Rabbit Anti-NECTIN2 Monoclonal Antibody (JG39-64) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK496 | Rabbit Anti-NECTIN2 Recombinant Antibody (clone JG39-64) | WB, ELISA | Rabbit IgG |
| VS3-FY1020 | Recombinant Rabbit Anti-NECTIN2 Antibody (clone R07-7G6) | WB | Rabbit IgG |
| VS13-YC780 | Rabbit Anti-NECTIN2 Recombinant Antibody (VS13-YC780) | IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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