Recombinant Rabbit Anti-NEDD4L Monoclonal Antibody (CBACN-396)
CAT#: MRO-1071-CN
This antibody is a rabbit monoclonal antibody that is capable of binding to NEDD4L.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human colon
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24618/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24618/55494_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24618/55494_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24618/55494_A_8_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24618/55494_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000049759-NEDD4L
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Specifications
- Immunogen
- N terminal of human NEDD4-2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human
- Species Reactivity
- Human, Mouse, Rat
- Clone
- CBACN-396
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like, E3 Ubiquitin Protein Ligase; HECT-Type E3 Ubiquitin Transferase NED4L; NEDD4-2; NEDD4.2; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like; E3 Ubiquitin-Protein Ligase NEDD4-Like; Ubiquitin-Protein Ligase Rsp5; EC 2.3.2.26;
- Gene ID
- 23327
- UniProt ID
- Q96PU5
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Efficient Immunoprecipitation
We used clone CBACN-396 for immunoprecipitation of NEDD4L from cell extracts. It worked efficiently, pulling down the E3 ubiquitin ligase for further analysis. The antibody's affinity for the N-terminal region seems very strong, and it reacts well with both human and mouse samples.
Clear Western Blot Bands
This rabbit monoclonal antibody provided clear and specific bands in our Western blot experiments. It showed broad reactivity across human, mouse, and rat lysates, which is excellent for our comparative research. The Protein A affinity purification ensures a clean signal with minimal background.
Q&As
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What is the immunogen used for this NEDD4L antibody?
A: The antibody (clone CBACN-396) was produced using the N-terminal region of human NEDD4-2 as the immunogen. It is a recombinant Rabbit Monoclonal Antibody that reacts with Human, Mouse, and Rat samples.
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What immunoassay techniques is this antibody used for?
A: This antibody is used for several immunoassay techniques, including Western Blot (WB) and Immunoprecipitation (IP). It is purified by Protein A affinity chromatography and is supplied in a TBS/BSA/Glycerol buffer.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "NEDD4L"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1330MZ | Recombinant Mouse Anti-Human NEDD4L Antibody | IHC-P, IP, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2408 | Hi-Affi™ Recombinant Rabbit Anti-NEDD4L Monoclonal Antibody (DS2408AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1108 | Rabbit Anti-NEDD4L Recombinant Antibody (VS3-WK1108) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-FY1460 | Human Anti-NEDD4L Recombinant Antibody (clone 2506W1355) | ELISA, Spiked IP, IF | Human IgG1 |
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