Mouse Anti-NEDD9 Recombinant Antibody (clone 3F7A9) (CAT#: VS7-0425-WR461)

• Immunogen
• Recombinant human NEDD9 (AA: 82-398) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human NEDD9

• Species Reactivity
• Human

• Clone
• 3F7A9

• Molecule Class
• Crk-Associated Substrates Family

• Applications
• WB, ICC, FC

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunocytochemistry: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• NEDD9; NEDD-9; Renal Carcinoma Antigen NY-REN-12; HEF1; CASS2; Cas-Like; Neural Precursor Cell Expressed, Developmentally Down-Regulated 9; Crk-Associated Substrate Related Protein Cas-L; CASL; Neural Precursor Cell Expressed Developmentally Down-Regulate

• Gene ID
• 4739

• UniProt ID
• Q14511

• Long Name
• Neural precursor cell expressed, developmentally down-regulated 9

• Cellular Localization
• Cell junction; Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Golgi apparatus; Membrane; Nucleus

• Post Translation Modifications
• Phosphoprotein; Ubl conjugation

• Protein Refseq
• NP_001135865.1

• Function
• Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (PubMed:24574519).; As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity).; May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form (PubMed:9020138).; Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs (PubMed:17174122).; Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse (By similarity).; Negatively regulates cilia outgrowth in polarized cysts (By similarity).; Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed:17604723).; Positively regulates RANKL-induced osteoclastogenesis (By similarity).; Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory (By similarity).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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