CytoStream™ Mouse Anti-NEFL Recombinant Antibody (VS13-YC782) (CAT#: VS13-YC782)
The CytoStream™ Mouse Anti-NEFL Recombinant Antibody is specific for human NEFL. NEFL encodes a light chain neurofilament protein, sustaining neuronal structure through axoskeleton support, with mutations linked to neuropathies like Charcot-Marie-Tooth disease. The anti-NEFL antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, intermediate filaments & midbody.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15021/1910_D3_1_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, intermediate filaments & midbody.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15021/1910_D3_1_selected.jpg
Figure 2 Low expression in tonsil
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_A_6_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_A_6_8_val_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_B_8_5.jpg
(Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_B_8_5.jpg
Figure 4 RNA cell line category: Group enriched (HEK93, PC-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000277586-NEFL
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000277586-NEFL
Specifications
- Immunogen
- Recombinant human NEFL expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NEFL
- Species Reactivity
- Human
- Applications
- WB, IHC-P, FC, ELISA
- Conjugate
- NONE
- Related Disease
- Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E)
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/100-1/200
Flow cytometry (FC): 1/50-1/100
ELISA: 1/10000
Target
- Alternative Names
- Neurofilament Light; Protein Phosphatase 1, Regulatory Subunit 110; Neurofilament, Light Polypeptide 68kDa; Neurofilament Triplet L Protein; NF-L; NF68; NFL; Light Molecular Weight Neurofilament Protein; Neurofilament Protein, Light Chain;
- Gene ID
- 4747
- UniProt ID
- P07196
- Long Name
- Neurofilament Light
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cell projection; Cytoplasm; Cytoskeleton; Intermediate filament
- Protein Refseq
- NP_006149.2
- Function
- Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NEFL"
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Chicken IgY Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0822MZ | Chicken Anti-NEFL Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1153MZ | Chicken Anti-Neurofilament Light Polyclonal IgY | WB, ICC | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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