Rabbit Anti-NEK9 Recombinant Antibody (clone R03-1F1) (CAT#: VS3-FY1797)

• Immunogen
• Synthetic peptide of human NEK9

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Rat NEK9

• Species Reactivity
• Human, Rat

• Clone
• R03-1F1

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence

• MW
• The calculated weight of the target protein is approximately 107 kDa, while the observed molecular weight stands at 107 kDa.

• Related Disease
• Nevus Comedonicus and Arthrogryposis, Perthes Disease, And Upward Gaze Palsy

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunofluorescence: 1/50-1/200

• Alternative Names
• NIMA Related Kinase 9; NIMA (Never In Mitosis Gene A)- Related Kinase 9; NimA-Related Protein Kinase 9; Nercc1 Kinase; EC 2.7.11.1; NERCC; Serine/Threonine-Protein Kinase Nek9; Never In Mitosis A-Related Kinase 9

• Gene ID
• 91754

• UniProt ID
• Q8TD19

• Sequence Similarities
• Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Autophosphorylated on serine and threonine residues (PubMed:27153399).
  When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210.
  During mitosis, not phosphorylated on Thr-210.
  Phosphorylated by CDK1 in vitro (PubMed:14660563).
  (Q8TD19-NEK9_HUMAN)
  Ubiquitination at Lys327, Lys606, and Lys650 (NX_Q8TD19 [NX_Q8TD19-1])
  Modification sites at PhosphoSitePlus (Q8TD19)
  Glycosylation from GlyGen (Q8TD19) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• XP_005268266.1; XP_047287875.1; XP_054232953.1; XP_054232954.1; NP_001316166.1

• Function
• Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation.
  Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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