Rabbit Anti-NEK9 Recombinant Antibody (clone R03-1F1)
CAT#: VS3-FY1797
This R03-1F1 recombinant monoclonal antibody has the ability to specifically bind to human NEK9. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The specificity of the NEK9 antibody was confirmed by western blot analysis of NEK9 in Hela lysates.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human testis
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1405/4531_A_5_6_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1405/4531_B_7_5.jpg
Normal Tissue
Figure 3 Rectum
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1405/4531_A_4_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1405/4531_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1405/4531_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1405/4531_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119638-NEK9
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WB
Figure 1 Anti-NEK9 Antibody (VS3-FY1797) in WB.
The NEK9 expression in Hela lysates were detected using the anti-NEK9 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human NEK9
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat NEK9
- Species Reactivity
- Human, Rat
- Clone
- R03-1F1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 107 kDa, while the observed molecular weight stands at 107 kDa.
- Related Disease
- Nevus Comedonicus and Arthrogryposis, Perthes Disease, And Upward Gaze Palsy
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- NIMA Related Kinase 9; NIMA (Never In Mitosis Gene A)- Related Kinase 9; NimA-Related Protein Kinase 9; Nercc1 Kinase; EC 2.7.11.1; NERCC; Serine/Threonine-Protein Kinase Nek9; Never In Mitosis A-Related Kinase 9
- Gene ID
- 91754
- UniProt ID
- Q8TD19
- Sequence Similarities
- Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Autophosphorylated on serine and threonine residues (PubMed:27153399).
When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210.
During mitosis, not phosphorylated on Thr-210.
Phosphorylated by CDK1 in vitro (PubMed:14660563).
(Q8TD19-NEK9_HUMAN)
Ubiquitination at Lys327, Lys606, and Lys650 (NX_Q8TD19 [NX_Q8TD19-1])
Modification sites at PhosphoSitePlus (Q8TD19)
Glycosylation from GlyGen (Q8TD19) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005268266.1; XP_047287875.1; XP_054232953.1; XP_054232954.1; NP_001316166.1
- Function
- Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation.
Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NEK9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1180CT | Recombinant Mouse anti-Human NEK9 Monoclonal antibody (EML2037) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2419 | Hi-Affi™ Recombinant Rabbit Anti-NEK9 Monoclonal Antibody (DS2419AB) | WB, IHC, ICC, FC | IgG |
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