Rabbit Anti-NFIB Recombinant Antibody (clone R03-0E5)
CAT#: VS3-FY1800
This R03-0E5 recombinant monoclonal antibody is specifically designed to target human NFIB. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IP. The binding specificity of the NFIB antibody was determined using western blot analysis of NFIB in C6, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/28_H11_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong nuclear positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/10623_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/10623_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/10623_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3956/10623_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (AN3-CA, HBEC3-KT, HEL, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147862-NFIB
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WB
Figure 1 Anti-NFIB Antibody (VS3-FY1800) in WB.
The NFIB expression in C6, Hela lysates were detected using the anti-NFIB by western blot assay.
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Specifications
- Immunogen
- Synthetic peptides of human NFIB
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat NFIB
- Species Reactivity
- Human, Rat
- Clone
- R03-0E5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 47,55 kDa.
- Related Disease
- Macrocephaly, Acquired, With Impaired Intellectual Development and Adenoid Cystic Carcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Nuclear Factor I B; CCAAT-Box-Binding Transcription Factor; TGGCA-Binding Protein; Nuclear Factor I/B; Nuclear Factor 1/B; NF-I/B; NF1-B; NFI-B
- Gene ID
- 4781
- UniProt ID
- O00712
- Sequence Similarities
- Belongs to the CTF/NF-I family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (O00712)
Modification sites at neXtProt (NX_O00712)
Glycosylation from GlyGen (O00712) 4 sites, 1 O-linked glycan (4 sites)
- Protein Refseq
- NP_001177666.1; NP_001177667.1; NP_001269716.1; NP_001356387.1; NP_001356388.1
- Function
- Transcriptional activator of GFAP, essential for proper brain development.
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NFIB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1710MZ | Recombinant Mouse Anti-Human NFIB Antibody | ELISA, WB | Mouse antibody |
| MRO-1085-CN | Recombinant Mouse Anti-NFIB Monoclonal Antibody (1H2) | WB, IHC, IF, FC | Mouse IgG2a |
| MRO-1086-CN | Recombinant Mouse Anti-NFIB Monoclonal Antibody (1H4) | WB, IHC, FC | Mouse IgG1 |
| MRO-1087-CN | Recombinant Mouse Anti-NFIB Monoclonal Antibody (1H5) | WB, IHC, FC | Mouse IgG2b |
| MRO-1088-CN | Recombinant Mouse Anti-NFIB Monoclonal Antibody (1H1) | IHC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4813 | Anti-NFIB Immunohistochemistry Kit | IHC | |
| VS-0525-XY4814 | Anti-Mouse NFIB Immunohistochemistry Kit | IHC |
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