Mouse Anti-NFKB2 Recombinant Antibody (clone 5E6A12)
CAT#: VS7-0425-WR477
This product is a mouse IgG1 antibody that binds to human NFKB2. The antibody 5E6A12 recognizes specific amino acid domain of human NFKB2 (AA: 712-900). It can be used for WB and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong positivity in enocrine and exocrine cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/if_selected.jpg
Normal Tissue
Figure 4 Appendix
High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_1_2_val_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_7_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077150-NFKB2
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Specifications
- Immunogen
- Recombinant human NFKB2 (AA: 712-900) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NFKB2
- Species Reactivity
- Human, Mouse
- Clone
- 5E6A12
- Molecule Class
- NF-κB Transcription Factor
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency, common variable, 10 (CVID10)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NFKB2; LYT-10; NFKB, P52/P100 Subunit; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells 2; Nuclear Factor NF-Kappa-B P52 Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2; CVID10; Transcription Factor NFKB2; Nuclear
- Gene ID
- 4791
- UniProt ID
- Q00653
- Long Name
- Nuclear Factor Kappa B Subunit 2
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001070962.1
- Function
- Activator; DNA-binding; Repressor
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
NLR Signaling Pathway
TCR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "NFKB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4053z | Mouse Anti-NFKB2 Recombinant Antibody (clone 29B2) | WB, IP, IF, FC | Mouse IgG |
| MRO-1089-CN | Recombinant Rabbit Anti-NFKB2 Monoclonal Antibody (JM82-03) | WB, IF, IHC, IP | Rabbit IgG |
| FAMAB-1228CQ | Mouse Anti-NFKB2 Recombinant Antibody (clone SAIC-26C-15) | Immuno-MRM, ELISA | Mouse IgG1 |
| VS13-YC798 | Rabbit Anti-NFKB2 Recombinant Antibody (VS13-YC798) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR478 | Mouse Anti-NFKB2 Recombinant Antibody (clone 2F10A7) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2434 | Hi-Affi™ Recombinant Rabbit Anti-NFKB2 Monoclonal Antibody (DS2434AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1495 | Anti-NFKB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4819 | Anti-Mouse NFKB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4818 | Anti-Human NFKB2 Immunohistochemistry Kit | IHC |
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