Mouse Anti-NHP ACTB Recombinant Antibody (VS-1024-XY53)
CAT#: VS-1024-XY53
Showcasing a mouse-derived monoclonal antibody, this reagent is tailored to target ACTB with remarkable reactivity across dog, hamster, human, mouse, non-human primate, and rat samples. The immunogen is a recombinant human ACTB protein, ensuring high specificity and reliable performance. Suitable for Western blot (WB) and immunohistochemistry (IHC) applications, this antibody excels in detecting ACTB expression across diverse species. Its broad reactivity and precision make it an indispensable tool for various research and diagnostic needs.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
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Specifications
- Immunogen
- Recombinant human ACTB protein
- Host Species
- Mouse
- Type
- Mouse IgG2b, kappa
- Specificity
- ACTB
- Species Reactivity
- Dog, Hamster, Human, Mouse, Non-human primate, Rat
- Applications
- WB, IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1-2 µg/mL, IHC: 1-2 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| MOR-0049 | Hi-Affi™ Rabbit Anti-ACTB Recombinant Antibody (clone DS49AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY28 | Anti-ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY114 | Anti-Mouse ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY113 | Anti-Human ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY115 | Anti-Rat ACTB Immunohistochemistry Kit | IHC |
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