Mouse Anti-NHP ACTB Recombinant Antibody (VS-1024-XY53) (CAT#: VS-1024-XY53)
Showcasing a mouse-derived monoclonal antibody, this reagent is tailored to target ACTB with remarkable reactivity across dog, hamster, human, mouse, non-human primate, and rat samples. The immunogen is a recombinant human ACTB protein, ensuring high specificity and reliable performance. Suitable for Western blot (WB) and immunohistochemistry (IHC) applications, this antibody excels in detecting ACTB expression across diverse species. Its broad reactivity and precision make it an indispensable tool for various research and diagnostic needs.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
Figure 5 Colon
(Enterocytes - Microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
(Enterocytes - Microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
Specifications
- Immunogen
- Recombinant human ACTB protein
- Host Species
- Mouse
- Type
- Mouse IgG2b, kappa
- Specificity
- ACTB
- Species Reactivity
- Dog, Hamster, Human, Mouse, Non-human primate, Rat
- Applications
- WB, IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1-2 µg/mL, IHC: 1-2 µg/mL
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-055 | Recombinant Anti-human ACTB VHH Single Domain Antibody | WB, ELISA, IP, FC, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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