Rat Anti-NHP AR Recombinant Antibody (clone AN1-15)
CAT#: VS-1024-XY24
This antibody is derived from rat and specifically targets the androgen receptor (AR). It demonstrates broad reactivity across human, mouse, non-human primates, and rat species. The immunogen used is a fusion protein of the human androgen receptor. With validated applications in Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF), this antibody provides versatile utility for detecting AR expression across multiple experimental contexts, making it ideal for both basic and translational research in androgen receptor-related pathways.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4733/1914_G6_27_cr5c8779fe8d0bf_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1/135_A_6_6.jpg
Normal Tissue
Figure 3 High expression in seminal vesicle.
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1/135_A_9_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, BJ hTERT+, HAP1, LHCN-M2, RH-30, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169083-AR
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Specifications
- Immunogen
- A fusion protein of human androgen receptor
- Host Species
- Rat
- Type
- Rat IgG
- Specificity
- AR
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- AN1-15
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:50-1:500, IHC: 3 µg/mL, IF: 5 µg/mL
Target
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Related Diseases
Prostate Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0226 | Hi-Affi™ Rabbit Anti-AR Recombinant Antibody (clone DS226AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0307F | Mouse Anti-AR Recombinant Antibody (ZG-0307F) | WB, ELISA | Mouse IgG |
| ZG-0308F | Mouse Anti-AR Recombinant Antibody (ZG-0308F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0548J | Rabbit Anti-AR Recombinant Antibody (clone 5G7) | ELISA, WB, IHC | Rabbit IgG |
| ZG-0549J | Rabbit Anti-AR Recombinant Antibody (clone 10A5) | ELISA, IHC | Rabbit IgG |
| VS13-YC62 | Rabbit Anti-AR Recombinant Antibody (VS13-YC62) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY143 | Anti-AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY485 | Anti-Mouse AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY484 | Anti-Human AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY486 | Anti-Monkey AR Immunohistochemistry Kit | IHC |
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