Mouse Anti-NHP ARFGAP1 Recombinant Antibody (clone OTI1F6) (CAT#: VS-1024-XY33)
The mouse-derived monoclonal antibody is highly specific for ARFGAP1, with proven reactivity in human and non-human primate samples. The antibody was generated using a full-length recombinant human ARFGAP1 protein as the immunogen, ensuring exceptional specificity. It is validated for a variety of applications, including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry (FC). This broad utility makes it an invaluable tool for detailed studies of ARFGAP1 expression and function across diverse experimental settings.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, cytosol, the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51019/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, cytosol, the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51019/if_selected.jpg
Figure 2 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/if_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/if_selected.jpg
Figure 3 IHC staining of human cervix, uterine
(Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/ihc_selected.jpg
(Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/ihc_selected.jpg
Figure 4 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/148206_B_8_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/148206_B_8_5.jpg
Figure 5 Cervix
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/148206_B_8_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56273/148206_B_8_3.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101199-ARFGAP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101199-ARFGAP1
Specifications
- Immunogen
- Full length human recombinant protein of ARFGAP1
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- ARFGAP1
- Species Reactivity
- Human, Non-human primate
- Clone
- OTI1F6
- Applications
- WB, IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800-1:20, IHC: 1:200, IF: 1:100, FC: 1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0232 | Hi-Affi™ Rabbit Anti-ARFGAP1 Recombinant Antibody (clone DS232AB) | WB, IF | Rabbit IgG |
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